The movement of coiled bodies visualized in living plant cells by the green fluorescent protein
| Autor: | Kurt Boudonck, Liam Dolan, Peter Shaw |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Transcription
Genetic Nucleolus Recombinant Fusion Proteins Green Fluorescent Proteins Arabidopsis Biology Article Gene Expression Regulation Plant Plant Cells Tobacco Organelle medicine Phosphorylation Molecular Biology Sequence Deletion Ribonucleoprotein Cell Nucleus Organelles Microscopy Confocal Nucleoplasm Cell Cycle Biological Transport Cell Biology Plants Ribonucleoprotein U2 Small Nuclear Cell biology Luminescent Proteins Plants Toxic Cell nucleus medicine.anatomical_structure Microscopy Fluorescence RNA splicing Coilin Nuclear transport |
| Popis: | Coiled bodies are nuclear organelles that contain components of at least three RNA-processing pathways: pre-mRNA splicing, histone mRNA 3′- maturation, and pre-rRNA processing. Their function remains unknown. However, it has been speculated that coiled bodies may be sites of splicing factor assembly and/or recycling, play a role in histone mRNA 3′-processing, or act as nuclear transport or sorting structures. To study the dynamics of coiled bodies in living cells, we have stably expressed a U2B"–green fluorescent protein fusion in tobacco BY-2 cells and in Arabidopsis plants. Time-lapse confocal microscopy has shown that coiled bodies are mobile organelles in plant cells. We have observed movements of coiled bodies in the nucleolus, in the nucleoplasm, and from the periphery of the nucleus into the nucleolus, which suggests a transport function for coiled bodies. Furthermore, we have observed coalescence of coiled bodies, which suggests a mechanism for the decrease in coiled body number during the cell cycle. Deletion analysis of the U2B"gene construct has shown that the first RNP-80 motif is sufficient for localization to the coiled body. |
| Databáze: | OpenAIRE |
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