New insights into tenocyte-immune cell interplay in an in vitro model of inflammation
| Autor: | Aysha Schmock, Franka Klatte-Schulz, Susann Minkwitz, Britt Wildemann, Martina Seifert, Meaghan Stolk |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Macrophage polarization lcsh:Medicine Inflammation Cell Communication Biology 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit Peripheral blood mononuclear cell Article Immunophenotyping 03 medical and health sciences 0302 clinical medicine Immune system Leukocytes medicine Humans Interferon gamma lcsh:Science Cells Cultured Multidisciplinary Macrophages lcsh:R Interleukin Cell biology Tenocytes 030104 developmental biology Cytokine Preclinical research 030220 oncology & carcinogenesis Immunology Leukocytes Mononuclear Cytokines lcsh:Q Tumor necrosis factor alpha Inflammation Mediators medicine.symptom Biomarkers medicine.drug |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Inflammation plays an important role in the development and resolution of tendon diseases, but underlying mechanisms are poorly understood. We therefore aimed to analyze the response of human tenocytes to inflammatory stimuli and to uncover their interplay with macrophages in vitro. Tenocytes from human ruptured supraspinatus tendons (n = 10) were treated for three days with a stimulation mixture derived from activated mononuclear cells isolated from healthy human peripheral blood. Significantly increased expression levels of selected adhesion- and human leukocyte antigen (HLA)-molecules, and enhanced interleukin (IL)-6 release were detected by flow cytometry. Tenocyte stimulation with the pro-inflammatory cytokines interferon gamma, tumor necrosis factor alpha and IL-1ß triggered similar changes in surface markers and enhanced the release of IL-6, IL-8 and monocyte chemoattractant protein 1 (MCP-1). In co-cultures of macrophages with pre-stimulated tenocytes, macrophages significantly increased CD80 expression, but simultaneously decreased HLA-DR-expression, which are both typical pro-inflammatory polarization markers. Co-cultures also released more IL-6, IL-8, MCP-1 than tenocyte-cultures alone. We demonstrate that tenocytes respond to inflammatory environments in vitro with altered surface marker and cytokine profiles and influence macrophage polarization. Importantly, all changes detected in direct co-cultures were also present in a transwell setting, implicating that communication between the cells involves soluble factors. |
| Databáze: | OpenAIRE |
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