The promotion of cardiogenic differentiation of hMSCs by targeting epidermal growth factor receptor using microRNA-133a
| Autor: | Chang Youn Lee, Woochul Chang, Onju Ham, Yangsoo Jang, Soyeon Lim, Byeong-Wook Song, Yoonjin Bae, Jun-Hee Park, Ki-Chul Hwang, Jiyun Lee, Hyang Hee Seo, Se-Yeon Lee, Min Ji Cha, Eunhyun Choi, Il-Kwon Kim, Eunmi Choi |
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| Rok vydání: | 2012 |
| Předmět: |
Male
endocrine system Heart disease Molecular Sequence Data Biophysics Myocardial Infarction Bioengineering CHO Cells Biomaterials Cell therapy Rats Sprague-Dawley Cricetinae medicine Animals Humans Luciferase Myocytes Cardiac Epidermal growth factor receptor Protein Kinase Inhibitors biology Base Sequence Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Transfection equipment and supplies medicine.disease Rats Transplantation ErbB Receptors MicroRNAs Mechanics of Materials Immunology Ceramics and Composites Cancer research biology.protein Stem cell Stem Cell Transplantation |
| Zdroj: | Biomaterials. 34(1) |
| ISSN: | 1878-5905 |
| Popis: | Human bone marrow-derived mesenchymal stem cells (hMSCs) are an attractive candidate for cell therapy in heart disease. Low survival and incomplete electromechanical integration between resident cardiomyocytes and transplanted hMSCs remain unsolved. In order for an infarcted heart to tolerate transplantation, differentiation capacity in stem cells must be reinforced. In this study, we found that compound 56, an epidermal growth factor receptor (EGFR) inhibitor, promotes cardiogenic differentiation of hMSCs and the transplantation of hMSCs treated with compound 56 resulted in enhancement of heart functions. Furthermore, hMSCs transfected with microRNA-133a (miR-133a), which targets EGFR, were observed to express cardiac-specific markers. We also discovered that luciferase activity is exclusively decreased by targeting EGFR in hMSCs transfected with miR-133a mimic. These results suggest that EGFR plays a key role in the regulation of cardiogenic differentiation in hMSCs. |
| Databáze: | OpenAIRE |
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