Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis
Autor: | Jeffrey Koury, Ana Ramirez, Chen Xie, Tom Ramos, Charli Dong, Chad Maki, Fari Izadyar, David Clark, Jerry Harb, Yvonne Drechsler, Jijun Hao, Gagandeep Kaur |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Male
Physiology Gene Expression medicine.disease_cause Biochemistry 0403 veterinary science Allergic sensitization White Blood Cells SH2B1 Animal Cells Immune Physiology Blood plasma Medicine and Health Sciences Medicine Dog Diseases Enzyme-Linked Immunoassays Mammals 0303 health sciences Innate Immune System Multidisciplinary T Cells Eukaryota 04 agricultural and veterinary sciences Atopic dermatitis Body Fluids Nucleic acids Blood Vertebrates Cytokines Female Cellular Types Anatomy Research Article 040301 veterinary sciences Science Immune Cells Immunology Research and Analysis Methods Peripheral blood mononuclear cell Blood Plasma Dermatitis Atopic 03 medical and health sciences Dogs Genetic predisposition Genetics Animals Immunoassays Non-coding RNA 030304 developmental biology Natural antisense transcripts Blood Cells business.industry Gene Expression Profiling Case-control study Organisms Biology and Life Sciences Cell Biology Immune dysregulation Molecular Development medicine.disease Gene regulation Cyclic Nucleotide Phosphodiesterases Type 4 MicroRNAs Immune System Case-Control Studies Amniotes Immunologic Techniques Leukocytes Mononuclear RNA business Biomarkers Developmental Biology |
Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 6, p e0218670 (2019) |
ISSN: | 1932-6203 |
Popis: | Canine Atopic Dermatitis (AD) is a common complex and multifactorial disease involving immune dysregulation, genetic predisposition, skin barrier defects, environmental factors and allergic sensitization. To date, diagnosis of canine AD relies on a combination of patient history, clinical examination, allergy testing and response to diet trials/therapies with no reliable biomarkers available to distinguish AD from other diseases with similar clinical presentations. A handful of studies to identify potential biomarkers in the peripheral blood of AD dogs and healthy controls have been performed with some showing inconsistent and contradictory results. In this study, we, for the first time, report statistically significant increases in expression of phosphodiesterase 4D (PDE4D) gene in peripheral blood mononuclear cells (PBMCs) and miR-203 in plasma from AD dogs compared to healthy controls. In addition, we report a statistically non-significant change of the CD4+/CD8+ ratio, a dramatic decrease of three gene markers (PIAS1, RORA and SH2B1) as well as a panel of differential expression of cytokines in AD dogs in comparison to the healthy controls. Our study provides important insight into the complexities of canine AD, and further studies to verify the specificity of these findings for canine AD at a larger-scale are warranted. |
Databáze: | OpenAIRE |
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