Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis

Autor: Jeffrey Koury, Ana Ramirez, Chen Xie, Tom Ramos, Charli Dong, Chad Maki, Fari Izadyar, David Clark, Jerry Harb, Yvonne Drechsler, Jijun Hao, Gagandeep Kaur
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
Physiology
Gene Expression
medicine.disease_cause
Biochemistry
0403 veterinary science
Allergic sensitization
White Blood Cells
SH2B1
Animal Cells
Immune Physiology
Blood plasma
Medicine and Health Sciences
Medicine
Dog Diseases
Enzyme-Linked Immunoassays
Mammals
0303 health sciences
Innate Immune System
Multidisciplinary
T Cells
Eukaryota
04 agricultural and veterinary sciences
Atopic dermatitis
Body Fluids
Nucleic acids
Blood
Vertebrates
Cytokines
Female
Cellular Types
Anatomy
Research Article
040301 veterinary sciences
Science
Immune Cells
Immunology
Research and Analysis Methods
Peripheral blood mononuclear cell
Blood Plasma
Dermatitis
Atopic
03 medical and health sciences
Dogs
Genetic predisposition
Genetics
Animals
Immunoassays
Non-coding RNA
030304 developmental biology
Natural antisense transcripts
Blood Cells
business.industry
Gene Expression Profiling
Case-control study
Organisms
Biology and Life Sciences
Cell Biology
Immune dysregulation
Molecular Development
medicine.disease
Gene regulation
Cyclic Nucleotide Phosphodiesterases
Type 4
MicroRNAs
Immune System
Case-Control Studies
Amniotes
Immunologic Techniques
Leukocytes
Mononuclear
RNA
business
Biomarkers
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 6, p e0218670 (2019)
ISSN: 1932-6203
Popis: Canine Atopic Dermatitis (AD) is a common complex and multifactorial disease involving immune dysregulation, genetic predisposition, skin barrier defects, environmental factors and allergic sensitization. To date, diagnosis of canine AD relies on a combination of patient history, clinical examination, allergy testing and response to diet trials/therapies with no reliable biomarkers available to distinguish AD from other diseases with similar clinical presentations. A handful of studies to identify potential biomarkers in the peripheral blood of AD dogs and healthy controls have been performed with some showing inconsistent and contradictory results. In this study, we, for the first time, report statistically significant increases in expression of phosphodiesterase 4D (PDE4D) gene in peripheral blood mononuclear cells (PBMCs) and miR-203 in plasma from AD dogs compared to healthy controls. In addition, we report a statistically non-significant change of the CD4+/CD8+ ratio, a dramatic decrease of three gene markers (PIAS1, RORA and SH2B1) as well as a panel of differential expression of cytokines in AD dogs in comparison to the healthy controls. Our study provides important insight into the complexities of canine AD, and further studies to verify the specificity of these findings for canine AD at a larger-scale are warranted.
Databáze: OpenAIRE
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