Optimized sensitive and inexpensive method to measure D-lactate as a surrogate marker of methylglyoxal fluxes in metabolically relevant contexts
| Autor: | Alejandro Gugliucci, Russell Caccavello |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
0303 health sciences
Measure (data warehouse) Chromatography Surrogate endpoint 030302 biochemistry & molecular biology Methylglyoxal Commercial kit Pyruvaldehyde General Biochemistry Genetics and Molecular Biology Orders of magnitude (mass) 03 medical and health sciences chemistry.chemical_compound chemistry Sepsis Gut permeability Humans Colorimetry Lactic Acid D lactate Molecular Biology Biomarkers Plate reader 030304 developmental biology |
| Zdroj: | Methods. 203:5-9 |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/j.ymeth.2020.06.010 |
| Popis: | Introduction Plasma D-lactate levels can be considered a surrogate indicator of MG flux. There are commercial methods specifically designed to cover pathological ranges (mmol/l in sepsis or leaky gut) that are not suitable to encompass the metabolically relevant ranges. Material and methods We modified and optimized the D-lactate Colorimetric Assay kit MAK058 from Sigma adding an ultracentrifugation step, kinetic reading and increasing reaction temperature. Results and conclusions We present a modified, optimized and validated method to measure serum D-lactate using a commercial kit to increase the sensitivity of the method to 2 orders of magnitude lower as well as minimizing interferences so that it may be used by researchers to explore the D-lactate pathway in metabolic disorders at a low cost (colorimetric method and a plate reader) and with a much higher specificity. |
| Databáze: | OpenAIRE |
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