Alterations of erythrocyte rheology and cellular susceptibility in end stage renal disease: Effects of peritoneal dialysis
| Autor: | Nesrin Zeynep Ertan, Mukaddes Sinan, Elif Ugurel, Semra Bozfakioglu, Ozlem Yalcin |
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| Přispěvatelé: | Uğurel, Elif, Yalçın, Özlem (ORCID 0000-0001-5547-6653 & YÖK ID 218440), Ertan, Nesrin Zeynep, Bozfakioglu, Semra, Sinan, Mukaddes, School of Medicine, Department of Physiology |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
Erythrocytes Physiology Blood viscosity 030232 urology & nephrology lcsh:Medicine 030204 cardiovascular system & hematology Hematocrit Physical Chemistry Biochemistry Erythrocyte aggregation Antioxidants Osmotic gradient ektacytometry Oxidative stress Replacement therapy Elongation index Analyzer lorca Hemodialysis Deformability Failure Erythropoietin Efficiency Lipid peroxidation chemistry.chemical_compound 0302 clinical medicine Animal Cells Materials Physics Malondialdehyde Red Blood Cells Medicine and Health Sciences Erythrocyte deformability lcsh:Science Shear Stresses chemistry.chemical_classification Multidisciplinary medicine.diagnostic_test Viscosity Chemistry Physics Glutathione peroxidase Classical Mechanics Hematology Middle Aged Blood Viscosity Catalase Blood proteins Body Fluids Blood Nephrology Medicine Multidisciplinary sciences Physical Sciences Mechanical Stress Female Cellular Types Anatomy Peritoneal Dialysis Research Article Adult medicine.medical_specialty Materials Science Blood Plasma End stage renal disease Young Adult 03 medical and health sciences Internal medicine Medical Dialysis Osmotic Shock medicine Humans Glutathione Peroxidase Blood Cells Superoxide Dismutase lcsh:R Biology and Life Sciences Cell Biology Oxidative Stress Endocrinology Chemical Properties Kidney Failure Chronic lcsh:Q |
| Zdroj: | PLoS ONE, Vol 12, Iss 2, p e0171371 (2017) PLOS One PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | In this study, we investigated the effects of peritoneal dialysis on hemorheological and hematological parameters and their relations with oxidant and antioxidant status of uremic patients. Hemorheological parameters (erythrocyte deformability, erythrocyte aggregation, osmotic deformability, blood and plasma viscosity) were measured in patients with renal insufficiency undergoing peritoneal dialysis (PD) and volunteers. Erythrocyte deformability, osmotic deformability and aggregation in both autologous plasma and 3% dextran 70 were measured by laser diffraction ektacytometry. Enzyme activities of glutathione peroxidase, superoxide dismutase and catalase were studied in erythrocytes; lipid peroxidation was studied by measuring the amount of malondialdehyde in both erythrocytes and plasma samples. Blood viscosity at native hematocrit was significantly lower in PD patients at all measured shear rates compared to controls, but it was high in PD patients at corrected (45%) hematocrit. Erythrocyte deformability did not show any difference between the two groups. Osmotic deformability was significantly lower in PD patients compared to controls. Aggregation index values were significantly high in PD patients in plasma Catalase and glutathione peroxidase activities in erythrocytes were decreased in PD patients whereas superoxide dismutase activity was increased compared to controls. Malondialdehyde was significantly increased in erythrocytes and plasma samples of PD patients which also shows correlations with aggregation parameters. It has been concluded that erythrocytes in PD patients are more prone to aggregation and this tendency could be influenced by lipid peroxidation activity in patient's plasma. These results imply that uremic conditions, loss of plasma proteins and an increased risk of oxidative stress because of decreasing levels of antioxidant enzymes affect erythrocyte rheology during peritoneal dialysis. This level of distortion may have crucial effects, impairing the blood flow dynamics and causing inadequate microcirculatory perfusion. © 2017 Ertan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Seed Fund of Koç University; Research Foundation of Istanbul University |
| Databáze: | OpenAIRE |
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