Solution-mediated effect of bioactive glass in poly (lactic-co-glycolic acid)-bioactive glass composites on osteogenesis of marrow stromal cells
| Autor: | Gwendolen C. Reilly, Jun Yao, Paul Ducheyne, Phoebe S. Leboy, Shula Radin |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Bone sialoprotein Stromal cell Materials science Polymers Cell Culture Techniques Biomedical Engineering Bone Marrow Cells macromolecular substances Article law.invention Biomaterials chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Osteogenesis law Animals Humans Lactic Acid RNA Messenger Composite material Cells Cultured Glycolic acid biology Mesenchymal stem cell technology industry and agriculture Metals and Alloys Alkaline Phosphatase Rats PLGA chemistry Bioactive glass Ceramics and Composites Osteocalcin biology.protein Alkaline phosphatase Female Stromal Cells Biomarkers Polyglycolic Acid |
| Zdroj: | Journal of Biomedical Materials Research Part A. :794-801 |
| ISSN: | 1552-4965 1549-3296 |
| Popis: | A previous study demonstrated that the incorporation of bioactive glass (BG) into poly (lactic-co-glycolic acid) (PLGA) can promote the osteoblastic differentiation of marrow stromal cells (MSCs) on PLGA by promoting the formation of a calcium–phosphate-rich layer on its surface. To further understand the mechanisms underlying the osteogenic effect of PLGA-BG composite scaffolds, whether solution-mediated factors derived from composite scaffolds/hybrids can promote osteogenesis of marrow stromal cells was tested. The dissolution product from PLGA-30%BG scaffold stimulated osteogenesis of MSCs, as was confirmed by increased mRNA expression of osteoblastic markers such as osteocalcin (OCN), alkaline phosphatase (ALP), and bone sialoprotein (BSP). The three-dimensional structure of the scaffolds may contribute to the production of cell-derived factors that promoted distant MSC differentiation. Thus PLGA-BG composites demonstrate significant potential as a bone-replacement material. |
| Databáze: | OpenAIRE |
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