The effect of supplementation of grape seed proanthocyanidin extract on vascular dysfunction in experimental diabetes
| Autor: | Muaz Belviranli, Hatice Baysal, Ayşe Saide Şahin, Nilsel Okudan, Hakkı Gökbel, Hulagu Bariskaner |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Nitroprusside Medicine (miscellaneous) Aorta Thoracic Pharmacology In Vitro Techniques medicine.disease_cause Antioxidants Diabetes Mellitus Experimental Rats Sprague-Dawley Diabetes mellitus Malondialdehyde Medicine Animals Proanthocyanidins Nutrition and Dietetics Grape Seed Extract business.industry Superoxide Dismutase medicine.disease Acetylcholine Rats Vascular endothelium Oxidative Stress Biochemistry Dietary Supplements Grape seed proanthocyanidin Endothelium Vascular Lipid Peroxidation business Oxidative stress Experimental diabetes |
| Zdroj: | Journal of medicinal food. 14(11) |
| ISSN: | 1557-7600 |
| Popis: | Increased oxidative stress and impaired endothelium-dependent relaxation could underlie many of the vascular complications associated with diabetes. We aimed to investigate the effect of supplementation with grape seed proanthocyanidin extract (GSPE), a natural antioxidant, on vascular responses and oxidative stress in streptozotocin-induced diabetic rats. Male Sprague-Dawley rats were divided into three groups: control rats, untreated diabetic rats, and GSPE (100 mg/kg, for 6 weeks)-supplemented diabetic rats. Thoracic aorta rings of the rats were mounted in organ baths, and relaxant responses to acetylcholine (ACh), A23187, and sodium nitroprusside (SNP) were assayed in tissues precontracted with 60 mM KCl. Plasma samples used for the measurement of malondialdehyde (MDA) level and superoxide dismutase (SOD) activity. The endothelium-dependent relaxations in response to ACh and A23187 were impaired, but endothelium-independent relaxation in response to SNP did not change in diabetic rats. Supplementation with GSPE significantly improved the relaxant responses to ACh and A23187. The MDA level was significantly elevated and the plasma SOD activity was decreased in diabetic rats, but supplementation with GSPE attenuated the elevated MDA levels and increased plasma SOD activity. Thus supplementation of GSPE may attenuate oxidative stress through the inhibition of lipid peroxidation and may restore endothelial function and reduce the risk of vascular disease in diabetes. |
| Databáze: | OpenAIRE |
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