Changes in Caenorhabditis elegans gene expression following exposure to Photorhabdus luminescens strain TT01
| Autor: | Amanda C. Wollenberg, Megan E. Hoinville |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Insecta Immunology Infections 03 medical and health sciences Enterobacteriaceae Species Specificity Photorhabdus luminescens Gene expression Animals Caenorhabditis elegans Symbiosis Gene Genetics biology Strain (biology) Gene Expression Regulation Developmental Biodiversity Heterorhabditis biology.organism_classification 030104 developmental biology Nematode Larva Photorhabdus Transcriptome Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 82:165-176 |
| ISSN: | 0145-305X |
| Popis: | Photorhabdus bacteria enter into a mutualistic symbiosis with Heterorhabditis nematodes to infect insect larvae. However, they rapidly kill the model nematode Caenorhabditis elegans. One hypothesis for these divergent outcomes is that the nematode defense responses differ. To begin testing this hypothesis, we have systematically analyzed available data on the transcriptional response of C. elegans to P. luminescens strain Hb. From a starting pool of over 7000 differentially expressed genes, we carefully chose 21 Heterorhabditis-conserved genes to develop as comparative markers. Using newly designed and validated qRT-PCR primers, we measured expression of these genes in C. elegans exposed to the sequenced TT01 strain of P. luminescens, on two different media types. Almost all (18/21) of the genes showed a significant response to P. luminescens strain TT01. One response is dependent on media type, and a subset of genes may respond differentially to distinct strains. Overall, we have established useful resources and generated new hypotheses regarding how C. elegans responds to P. luminescens infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect