Characterization of tissue specific differences in cell wall polysaccharides of ripe and overripe pear fruit
| Autor: | Catherine M.G.C. Renard, Sylvie Bureau, Carine Le Bourvellec, Marwa Brahem, Barbara Gouble |
|---|---|
| Přispěvatelé: | Le Bourvellec, Carine, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ministry of Higher Education and Scientific Research of Tunisia RB3C-2476 |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
fermeté du fruit Polymers and Plastics Pectin [SDV]Life Sciences [q-bio] 01 natural sciences Lignin microscopie électronique à balayage séparation cellulaire Pyrus chemistry.chemical_compound Cell Wall [SDV.IDA]Life Sciences [q-bio]/Food engineering Materials Chemistry chemistry.chemical_classification PEAR biology Pyrus communis L Hemicellulose Cellulose Sequential extraction food and beverages Biochemistry extraction de pectine Pectins Xylans Medium infrared reflectance spectroscopy (MIR) cellule pierreuse paroi cellulaire Pyrus communis stade de maturite food.ingredient Polysaccharide Cell wall food Polysaccharides extraction séquentielle [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering pyrus communis 010401 analytical chemistry Organic Chemistry biology.organism_classification 0104 chemical sciences chemistry Fruit 010606 plant biology & botany |
| Zdroj: | Carbohydrate Polymers (156), 152-164. (2017) Carbohydrate Polymers Carbohydrate Polymers, Elsevier, 2017, 156, pp.152-164. ⟨10.1016/j.carbpol.2016.09.019⟩ |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2016.09.019⟩ |
| Popis: | International audience; Cell walls from flesh, parenchyma cells, stone cells and skin were isolated from ripe and overripe Pyrus communis L. cv “De Cloche” using the phenol-buffer method. Pear polysaccharides were solubilized from cell walls by sequential extractions with aqueous solutions of ammonium oxalate, Na2CO3, and increasing concentration of NaOH, to explore overripening impact. Cell walls were also differentiated using MIR spectral data. Stone cells contained high levels of xylose and lignin while parenchyma cells had high levels of glucose, uronic acids and arabinose. Sequential extractions revealed that pear pectins had highly branched rhamnogalacturonans and were extremely methylated. Xylans were the main hemicellulose especially for stone cells. Cellulose represented about half of all cell walls. This heterogeneous composition of pear affected differently cell wall evolutions and properties. Thus, overripening involved a decrease in arabinose and a loss of pectic side chains mostly from parenchyma cells. Changes in hemicellulose and cellulose were minor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect