| Autor: |
E A Borisova, Boris Zhivotovsky, Kaido P. Hanson, E A Zherbin, A B Chukhlovin, A. A. Seiliev |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine. 51:421-428 |
| ISSN: |
0020-7616 |
| DOI: |
10.1080/09553008714550911 |
| Popis: |
The relationship between nuclear chromatin degradation to polydeoxyribonucleotides (PDN) and other features of interphase death were studied using thymocytes of normal and X-irradiated rats. Fractionation of the thymic cells in Percoll gradients was performed in order to separate dead from intact cells. The degree of radiation-induced chromatin fragmentation, as assessed by electrophoresis, was similar for PDN from all Percoll bands. Following irradiation 87-98 per cent of 'heavy' thymocytes were pyknotic and almost devoid of receptors to autologous erythrocytes thus comprising a dead cell population. A direct relationship between PDN content and nuclear pyknosis was noted throughout the gradient. The loss of autologous rosette-forming ability was directly related to other indices of interphase death. The possibility of PDN originating from pyknosis-prone cells and the capacity of radiosensitive thymocytes to form autologous rosettes are discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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