Visualisation by vital staining with trypan blue of wounds punctured by Varroa destructor mites in pupae of the honey bee (Apis mellifera)
| Autor: | Wolf Engels, Ghazwan Kanbar |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
0106 biological sciences
Veterinary medicine animal structures trypan blue [SDV.BID]Life Sciences [q-bio]/Biodiversity 010603 evolutionary biology 01 natural sciences integumental puncture chemistry.chemical_compound Botany [SDV.EE]Life Sciences [q-bio]/Ecology environment integumentary system Apidae biology fungi Honey bee pupal infestation vital staining biology.organism_classification Brood Staining Apoidea [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology 010602 entomology Vital stain chemistry [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies Insect Science Varroa destructor Trypan blue Apis mellifera |
| Zdroj: | Apidologie Apidologie, Springer Verlag, 2004, 35 (1), pp.25-29. ⟨10.1051/apido:2003057⟩ |
| ISSN: | 1297-9678 0044-8435 |
| DOI: | 10.1051/apido:2003057 |
| Popis: | After invading a honey bee brood cell shortly before capping, female Varroa destructor mites puncture the integument of the host bee in order to suck haemolymph. The perforations used as feeding sites are difficult to detect. We developed a method of vital staining with trypan blue to visualise the wounds. The dye is taken up by damaged epidermal cells in the margin of repeatedly used punctures. This new coloration method allows localisation of wounds in prepupal and especially in all pupal stages of workers and of drones, but the staining failed in early postfeeding 5th instar larvae. The low-cost method is easy to use. Because secondary infestations by viral and bacterial pathogens are assumed to enter the bees through these wounds, the trypan blue method may be useful in studies on parasite-host relations, especially concerning vector functions. Since pupae survive the vital staining without apparent harm, they can be used subsequently for other investigations. Apis mellifera / Varroa destructor / pupal infestation / integumental puncture / vital staining / trypan blue |
| Databáze: | OpenAIRE |
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