Expression of Transcription Factor GATA-6 in Alveolar Epithelial Cells Is Linked to Neonatal Lung Disease
Autor: | Sture Andersson, Tiina M. Asikainen, Riitta Karikoski, Vuokko L. Kinnula, Riika Vähätalo, Marjukka Myllärniemi, Carl W. White, Markku Heikinheimo |
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Rok vydání: | 2010 |
Předmět: |
Male
In Vitro Techniques Biology behavioral disciplines and activities 03 medical and health sciences Transcription Factor GATA-6 Fetus 0302 clinical medicine GATA6 Transcription Factor mental disorders medicine Animals Humans Transcription factor 030304 developmental biology Regulation of gene expression Original Paper Respiratory Distress Syndrome Newborn 0303 health sciences Lung Respiratory distress Reverse Transcriptase Polymerase Chain Reaction Infant Newborn Gene Expression Regulation Developmental respiratory system medicine.disease Immunohistochemistry respiratory tract diseases 3. Good health DNA-Binding Proteins Pulmonary Alveoli medicine.anatomical_structure 030228 respiratory system Bronchopulmonary dysplasia Premature birth Pediatrics Perinatology and Child Health Immunology RNA Female Infant Premature Papio Transcription Factors Developmental Biology |
Zdroj: | Neonatology. 99:231-240 |
ISSN: | 1661-7819 1661-7800 |
Popis: | Background: Premature birth and respiratory distress syndrome (RDS) are risk factors for disturbed lung development and bronchopulmonary dysplasia (BPD). The molecular mechanisms related to prematurity and BPD remain largely unknown. Epithelial expression of the transcription factor GATA-6 has been implicated in normal and abnormal murine lung development. Objectives: The possible involvement of GATA-6 in the normal development and in RDS and BPD was investigated in the human and baboon lung. Methods: Immunohistochemistry was used to study the expression of GATA-6 and thyroid transcription factor 1 in lung specimens from different age groups of human and baboon fetuses and newborns with lung disease. Furthermore, the regulatory role of TGF-β1 in GATA-6 expression was investigated in human pulmonary epithelial cell lines using RT-PCR. Results: GATA-6 expression increased in the developing human airway epithelium along with advancing gestation, but diminished to negligible at birth. In RDS, GATA-6 expression was enhanced at 5–7 days after birth, and decreased thereafter. In BPD, the expression of GATA-6 in alveolar epithelial cells was low. These results were confirmed and extended using an established baboon model of prematurity. The in vitro experiments revealed that TGF-β1 induces GATA-6 and thyroid transcription factor 1 expression in lung epithelial cells. Conclusions: Our results suggest that the expression of GATA-6 at the early stages of the preterm lung may be related to impaired postnatal alveolar development. |
Databáze: | OpenAIRE |
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