Expression of Transcription Factor GATA-6 in Alveolar Epithelial Cells Is Linked to Neonatal Lung Disease

Autor: Sture Andersson, Tiina M. Asikainen, Riitta Karikoski, Vuokko L. Kinnula, Riika Vähätalo, Marjukka Myllärniemi, Carl W. White, Markku Heikinheimo
Rok vydání: 2010
Předmět:
Male
In Vitro Techniques
Biology
behavioral disciplines and activities
03 medical and health sciences
Transcription Factor GATA-6
Fetus
0302 clinical medicine
GATA6 Transcription Factor
mental disorders
medicine
Animals
Humans
Transcription factor
030304 developmental biology
Regulation of gene expression
Original Paper
Respiratory Distress Syndrome
Newborn
0303 health sciences
Lung
Respiratory distress
Reverse Transcriptase Polymerase Chain Reaction
Infant
Newborn
Gene Expression Regulation
Developmental
respiratory system
medicine.disease
Immunohistochemistry
respiratory tract diseases
3. Good health
DNA-Binding Proteins
Pulmonary Alveoli
medicine.anatomical_structure
030228 respiratory system
Bronchopulmonary dysplasia
Premature birth
Pediatrics
Perinatology and Child Health
Immunology
RNA
Female
Infant
Premature
Papio
Transcription Factors
Developmental Biology
Zdroj: Neonatology. 99:231-240
ISSN: 1661-7819
1661-7800
Popis: Background: Premature birth and respiratory distress syndrome (RDS) are risk factors for disturbed lung development and bronchopulmonary dysplasia (BPD). The molecular mechanisms related to prematurity and BPD remain largely unknown. Epithelial expression of the transcription factor GATA-6 has been implicated in normal and abnormal murine lung development. Objectives: The possible involvement of GATA-6 in the normal development and in RDS and BPD was investigated in the human and baboon lung. Methods: Immunohistochemistry was used to study the expression of GATA-6 and thyroid transcription factor 1 in lung specimens from different age groups of human and baboon fetuses and newborns with lung disease. Furthermore, the regulatory role of TGF-β1 in GATA-6 expression was investigated in human pulmonary epithelial cell lines using RT-PCR. Results: GATA-6 expression increased in the developing human airway epithelium along with advancing gestation, but diminished to negligible at birth. In RDS, GATA-6 expression was enhanced at 5–7 days after birth, and decreased thereafter. In BPD, the expression of GATA-6 in alveolar epithelial cells was low. These results were confirmed and extended using an established baboon model of prematurity. The in vitro experiments revealed that TGF-β1 induces GATA-6 and thyroid transcription factor 1 expression in lung epithelial cells. Conclusions: Our results suggest that the expression of GATA-6 at the early stages of the preterm lung may be related to impaired postnatal alveolar development.
Databáze: OpenAIRE
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