Liquid chromatography with pre-column dansyl derivatisation and fluorimetric detection applied to the assay of morphine in biological samples
| Autor: | R. Dorizzi, Franco Tagliaro, A. Frigerio, G. Lubli, Mario Marigo |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Fluorescence spectrometry
Nalorphine Urine Biochemistry High-performance liquid chromatography Mass Spectrometry Analytical Chemistry Drug Stability medicine Chromatography High Pressure Liquid Dansyl Compounds Detection limit Total blood Chromatography Chemistry Organic Chemistry morphine General Medicine Kinetics Spectrometry Fluorescence Morphine fluorimetric detection Extraction methods medicine.drug |
| Zdroj: | Journal of Chromatography A. 330:323-331 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(01)81989-0 |
| Popis: | A simple method employing pre-column dansylation and liquid chromatography is proposed for a very sensitive and specific assay of morphine in biological samples. Nalorphine is used as an internal standard. The detection limit is 0.2 picomol of injected morphine. In the assay of human sera spiked with 150 nmol/1, the intra- and inter-assay coefficients of variation were 3.7% (n 10) and 4.5% (n 10), respectively. No interferences were observed from more than 70 opiate and non-opiate drugs. Urine, plasma and total blood were assayed, using different extraction methods, with negligible interference from coextractives. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|