Linoleic acid-stimulated vascular adhesion molecule-1 expression in endothelial cells depends on nuclear factor-[kappa ]B activation
| Autor: | Audrey Niemann Jönson, Cecilia M. Giachelli, Jan Nilsson, Per Eriksson, Mikko P.S. Ares, Otmar Pachinger, Anders Hamsten, Wolfgang Dichtl, Stefan Jovinge |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Endothelium Endocrinology Diabetes and Metabolism Linoleic acid Fluorescent Antibody Technique Vascular Cell Adhesion Molecule-1 Enzyme-Linked Immunosorbent Assay Biology Linoleic Acid Endothelial activation chemistry.chemical_compound Endocrinology Internal medicine Plasminogen Activator Inhibitor 1 medicine Animals Humans RNA Messenger Aorta Cells Cultured chemistry.chemical_classification Reverse Transcriptase Polymerase Chain Reaction NF-kappa B Fatty acid Blotting Northern Eicosapentaenoic acid Rats Endothelial stem cell Vascular endothelial growth factor A medicine.anatomical_structure chemistry Docosahexaenoic acid Endothelium Vascular |
| Zdroj: | Metabolism. 51:327-333 |
| ISSN: | 0026-0495 |
| DOI: | 10.1053/meta.2002.29963 |
| Popis: | Endothelial activation is an important step in atherogenesis. In addition to established cardiovascular risk factors, such as hypercholesterolemia, hypertension, diabetes mellitus, and homocysteinemia, high plasma levels of triglyceride-rich lipoproteins may be an important cause of endothelial activation as well. Free fatty acids hydrolyzed from core triglycerides of these particles can exert both pro- and anti-inflammatory effects on the vascular wall. omega-3 fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have been shown to inhibit cytokine-induced endothelial activation. In contrast, we and others have previously shown that the omega-6 fatty acid linoleate activates transcription factor nuclear factor-kappaB (NF-kappaB) in endothelial cells. In this study, we show that linoleic acid stimulates vascular adhesion molecule-1 (VCAM-1) protein and mRNA expression in cultured human endothelial cells, as assessed by immunofluorescence and Northern blotting. Release of shedded soluble VCAM-1 from cultured human endothelial cells was also increased by the addition of linoleic acid, as determined by enzyme-linked immunosorbent assay (ELISA). By use of cultured rat aortic endothelial cells transfected with an IkappaB super-repressor (DeltaN2 cells), we provide evidence that NF-kappaB signalling is required in the linoleic acid-induced VCAM-1 expression in endothelial cells, whereas other transcription factors appear to be involved in the increased endothelial plasminogen activator inhibitor-1 (PAI-1) production in response to linoleic acid. These findings suggest that diets rich in linoleic acid may be proinflammatory and thus atherogenic by activating vascular endothelial cells. |
| Databáze: | OpenAIRE |
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