Cloning, functional expression, and mutational analysis of a cDNA for Lotus japonicus mitochondrial phosphate transporter
| Autor: | Shingo Hata, Kazuki Nakamori, Katsura Izui, Hiroshi Kouchi, Reona Takabatake, Yosuke Umehara |
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| Rok vydání: | 2002 |
| Předmět: |
Root nodule
DNA Complementary Physiology Lotus japonicus DNA Mutational Analysis Molecular Sequence Data Plant Science Mitochondrial Proteins Gene Expression Regulation Plant Complementary DNA Phosphate Transport Proteins Amino Acid Sequence Cloning Molecular Site-directed mutagenesis Gene Phylogeny Cloning biology Sequence Homology Amino Acid fungi Mutagenesis food and beverages Transporter Cell Biology General Medicine Sequence Analysis DNA biology.organism_classification Molecular biology Biochemistry Lotus Mutagenesis Site-Directed |
| Zdroj: | Plantcell physiology. 43(10) |
| ISSN: | 0032-0781 |
| Popis: | A full-length cDNA encoding mitochondrial phosphate transporter was isolated from symbiotic nodules of Lotus japonicus, a model legume. The gene appeared to be single-copied and its transcript was abundant in roots, symbiotic root nodules and shoots, in that order. The L. japonicus transporter has a conserved N-ethylmaleimide-reactive Cys residue. Site-directed mutagenesis and phosphate transport assaying in vitro showed that this Cys residue is preferable for the transport activity, although it can be replaced to some extent by Ser, but not by Ala. |
| Databáze: | OpenAIRE |
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