A Portable Biosensor for 2,4-Dinitrotoluene Vapors
| Autor: | Gernot Thomas John, Christian Ude, Marc Prante, Ulrich Krings, Miriam Große, Lukas Raddatz, Shimshon Belkin, Thomas Scheper |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
2 4-Dinitrotoluene synthesis Cost effectiveness 2 4-dinitrotoluene Dewey Decimal Classification::600 | Technik::620 | Ingenieurwissenschaften und Maschinenbau Explosive materials Biosensing Techniques lcsh:Chemical technology Biochemistry 2 4 6-trinitrotoluene Analytical Chemistry chemistry.chemical_compound Explosives detection lcsh:TP1-1185 Explosive material Instrumentation biological sensor Phosphorescence Biological sensors explosive bioluminescence Atomic and Molecular Physics and Optics Dinitrobenzenes landmine detection nitrobenzene derivative Oxygen penetration depth Agarose explosive material Gases ddc:620 Bioluminescence Portable biosensors Wireless Technology devices Materials science Biological sensor Nanotechnology Chemical vapor signature chemistry biosensor Article 03 medical and health sciences Explosive Agents gas Landmine detection Humans human Electrical and Electronic Engineering isolation and purification Biological materials 2 4-dinitrotoluene wireless communication Oxygen Molecular oxygen 030104 developmental biology Biosensors Bombs (ordnance) Cell immobilization Explosives Hazardous chemicals chemical vapor signature Chemical vapor Biosensor genetic procedures Oxygen penetration |
| Zdroj: | Sensors, Vol 18, Iss 12, p 4247 (2018) Sensors (Switzerland) 18 (2018), Nr. 12 Sensors (Basel, Switzerland) Sensors Volume 18 Issue 12 |
| ISSN: | 1424-8220 |
| Popis: | Buried explosive material, e.g., landmines, represent a severe issue for human safety all over the world. Most explosives consist of environmentally hazardous chemicals like 2,4,6-trinitrotoluene (TNT), carcinogenic 2,4-dinitrotoluene (2,4-DNT) and related compounds. Vapors leaking from buried landmines offer a detection marker for landmines, presenting an option to detect landmines without relying on metal detection. 2,4-Dinitrotoluene (DNT), an impurity and byproduct of common TNT synthesis, is a feasible detection marker since it is extremely volatile. We report on the construction of a wireless, handy and cost effective 2,4-dinitrotoluene biosensor combining recombinant bioluminescent bacterial cells and a compact, portable optical detection device. This biosensor could serve as a potential alternative to the current detection technique. The influence of temperature, oxygen and different immobilization procedures on bioluminescence were tested. Oxygen penetration depth in agarose gels was investigated, and showed that aeration with molecular oxygen is necessary to maintain bioluminescence activity at higher cell densities. Bioluminescence was low even at high cell densities and 2,4-DNT concentrations, hence optimization of different prototypes was carried out regarding radiation surface of the gels used for immobilization. These findings were applied to sensor construction, and 50 ppb gaseous 2,4-DNT was successfully detected. |
| Databáze: | OpenAIRE |
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