Plasmidome of an environmental Acinetobacter lwoffii strain originating from a former gold and arsenic mine
| Autor: | Tomasz Walter, Marcin Jurkowski, Joanna Klim, Jan Gawor, Iwona Köhling, Małgorzata Słodownik, Urszula Zielenkiewicz |
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| Rok vydání: | 2019 |
| Předmět: |
Genetics
Transposable element DNA Bacterial biology Acinetobacter Whole Genome Sequencing In silico Computational Biology Toxin-Antitoxin Systems biology.organism_classification Mining Arsenic Plasmid Bacterial Proteins DNA Transposable Elements Plasmidome Gold Mobile genetic elements Acinetobacter lwoffii Molecular Biology Gene Genome Bacterial Plasmids |
| Zdroj: | Plasmid. 110 |
| ISSN: | 1095-9890 |
| Popis: | Emerging important Acinetobacter strains commonly accommodate a plethora of mobile elements including plasmids of different size. Plasmids, apart from encoding modules enabling their self-replication and/or transmission, can carry a diverse number of genes, allowing the host cell to survive in an environment that would otherwise be lethal or restrictive for growth. The present study characterizes the plasmidome generated from an arsenic-resistant strain named ZS207, classified as Acinetobacter lwoffii. Sequencing effort revealed the presence of nine plasmids in the size between 4.3 and 38.4 kb as well as one 186.6 kb megaplasmid. All plasmids, except the megaplasmid, do apparently not confer distinguishing phenotypic features. In contrast, the megaplasmid carries arsenic and heavy metals resistance regions similar to those found in permafrost A. lwoffii strains. In-depth in silico analyses have shown a significant similarity between the regions from these plasmids, especially concerning multiple transposable elements, transfer and mobilization genes, and toxin-antitoxin systems. Since ars genes encode proteins of major significance in terms of potential use in bioremediation, arsenic resistance level of ZS207 was determined and the functionality of selected ars genes was examined. Additionally, we checked the functionality of plasmid-encoded toxin-antitoxin systems and their impact on the formation of persister cells. |
| Databáze: | OpenAIRE |
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