Fentanyl vapor self-administration model in mice to study opioid addiction
Autor: | Antonello Bonci, Stephanie C. Gantz, Maria M Ortiz, Renata C. N. Marchette, Brendan J. Tunstall, Khaled Moussawi, George F. Koob, Leandro F. Vendruscolo |
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Rok vydání: | 2020 |
Předmět: |
Drug
media_common.quotation_subject Drug-Seeking Behavior Diseases and Disorders Self Administration Pharmacology Fentanyl Mice 03 medical and health sciences 0302 clinical medicine Dopamine mental disorders medicine Animals Opioid addiction Research Articles 030304 developmental biology media_common 0303 health sciences Multidisciplinary business.industry Addiction technology industry and agriculture SciAdv r-articles Opioid-Related Disorders Analgesics Opioid Ventral tegmental area medicine.anatomical_structure Opioid Self-administration business 030217 neurology & neurosurgery Research Article Neuroscience medicine.drug |
Zdroj: | Science Advances |
ISSN: | 2375-2548 |
Popis: | Fentanyl vapor self-administration in mice provides a novel and noninvasive model to study opioid addiction. Intravenous drug self-administration is considered the “gold standard” model to investigate the neurobiology of drug addiction in rodents. However, its use in mice is limited by frequent complications of intravenous catheterization. Given the many advantages of using mice in biomedical research, we developed a noninvasive mouse model of opioid self-administration using vaporized fentanyl. Mice readily self-administered fentanyl vapor, titrated their drug intake, and exhibited addiction-like behaviors, including escalation of drug intake, somatic signs of withdrawal, drug intake despite punishment, and reinstatement of drug seeking. Electrophysiological recordings from ventral tegmental area dopamine neurons showed a lower amplitude of GABAB receptor–dependent currents during protracted abstinence from fentanyl vapor self-administration. This mouse model of fentanyl self-administration recapitulates key features of opioid addiction, overcomes limitations of the intravenous model, and allows investigation of the neurobiology of opioid addiction in unprecedented ways. |
Databáze: | OpenAIRE |
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