Identification of porcine reproductive and respiratory syndrome virus inhibitors through an oriented screening on natural products
| Autor: | LI Dian-peng, Dai Fei-yan, Yin Ge-fen, Cheng Yong-xian, Li WenGui, BI Jun-long |
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| Rok vydání: | 2012 |
| Předmět: |
Medical knowledge
Natural product Cytopathic effect biology animal diseases General Chemistry Articles Porcine reproductive and respiratory syndrome virus biology.organism_classification Virology Reverse transcription polymerase chain reaction chemistry.chemical_compound chemistry Respiratory system Porcine reproductive and respiratory syndrome virus(PRRSV) Antiviral activity Gene IC50 |
| Zdroj: | Chemical Research in Chinese Universities |
| ISSN: | 1005-9040 |
| Popis: | Porcine reproductive and respiratory syndrome(PRRS) caused by porcine reproductive and respiratory syndrome virus(PRRSV) is one of the most infectious diseases in the swine industry worldwide, causing big economic losses. Vaccines are major weapons against PRRSV, however, current available vaccines have several limitations. Developing chemical drugs as alternatives is required. On the basis of traditional medical knowledge, we purposely selected 15 natural products originated from Chinese herbs with anti-infectious effects. Their antiviral activities were evaluated by PRRSV-induced cytopathic effect(CPE) on MARC-145 cells and reverse transcription polymerase chain reaction(RT-PCR) assay. Compounds ethoxysanguinarine(EOSG) and atractylodinol were found to be the hits which could significantly reduce PRRSV-associated CPE with 50% inhibited concentration(IC50) values of 7.9 and 39.4 μmol/L, respectively. Meanwhile, compounds ethoxysanguinarine and atractylodinol significantly decreased mRNA expression of ORF7 gene in a dose-dependent manner. Study results suggest that compounds ethoxysanguinarine and atractylodinol may be useful anti-PRRSV drugs for swine industry or the hits for further lead optimization. |
| Databáze: | OpenAIRE |
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