Uterine epithelial expression of the tumor necrosis factor superfamily: a strategy for immune privilege during pregnancy in a true epitheliochorial placentation species
| Autor: | Cheol-Heui Yun, Minsun Hong, Inkyu Yoo, Hakhyun Ka, Soohyung Lee, Wonchul Jung, Jisoo Han, Yoon-Chul Kye, Minjeong Kim, Tae Sub Park |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Fas Ligand Protein Stromal cell Swine Placenta Estrous Cycle Immune Privilege Biology Endometrium Epithelium Fas ligand Immune tolerance TNF-Related Apoptosis-Inducing Ligand Andrology 03 medical and health sciences 0302 clinical medicine Immune system Immune privilege Pregnancy medicine Animals fas Receptor Uterus Placentation Cell Biology General Medicine Receptors TNF-Related Apoptosis-Inducing Ligand 030104 developmental biology medicine.anatomical_structure Reproductive Medicine 030220 oncology & carcinogenesis Female Tumor necrosis factor alpha |
| Zdroj: | Biology of Reproduction. 102:828-842 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1093/biolre/ioz233 |
| Popis: | The maternal immune system tolerates semi-allogeneic placental tissues during pregnancy. Fas ligand (FASLG) and tumor necrosis factor superfamily 10 (TNFSF10) are known to be components of maternal immune tolerance in humans and mice. However, the role of FASLG and TNFSF10 in the tolerance process has not been studied in pigs, which form a true epitheliochorial type placenta. Thus, the present study examined the expression and function of FASLG and TNFSF10 and their receptors at the maternal-conceptus interface in pigs. The endometrium and conceptus tissues expressed FASLG and TNFSF10 and their receptor mRNAs during pregnancy in a stage-specific manner. During pregnancy, FASLG and TNFSF10 proteins were localized predominantly to endometrial luminal epithelial cells with strong signals on Day 30 to term and on Day 15, respectively, and receptors for TNFSF10 were localized to some stromal cells. Interferon-γ (IFNG) increased the expression of TNFSF10 and FAS in endometrial tissues. Co-culture of porcine endometrial epithelial cells over-expressing TNFSF10 with peripheral blood mononuclear cells yielded increased apoptotic cell death of lymphocytes and myeloid cells. In addition, many apoptotic T cells were found in the endometrium on Day 15 of pregnancy. The present study demonstrated that FASLG and TNFSF10 were expressed at the maternal-conceptus interface and conceptus-derived IFNG increased endometrial epithelial TNFSF10, which, in turn, induced apoptotic cell death of immune cells. These results suggest that endometrial epithelial FASLG and TNFSF10 may be critical for the formation of micro-environmental immune privilege at the maternal-conceptus interface for the establishment and maintenance of pregnancy in pigs. |
| Databáze: | OpenAIRE |
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