Oxygen recovery up-regulates avian UCP and ANT in newly hatched ducklings

Autor: Marion Spée, Aurélie Girard, Maud Belouze, Josiane Prost, Damien Roussel, Claude Duchamp, Benjamin Rey
Přispěvatelé: Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2009
Předmět:
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
animal structures
Physiology
Oxidative phosphorylation
Mitochondrion
Biology
medicine.disease_cause
Biochemistry
Avian Proteins
Mitochondrial Proteins
Lipid peroxidation
Andrology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
medicine
Animals
Uncoupling protein
RNA
Messenger
Hypoxia
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Reactive oxygen species
Muscles
Hypoxia (medical)
ANT
Oxygen
Oxidative Stress
Ducks
Animals
Newborn
chemistry
embryonic structures
Mitochondrial Uncoupling Proteins
Animal Science and Zoology
Lipid Peroxidation
medicine.symptom
Reactive Oxygen Species
Mitochondrial ADP
ATP Translocases
030217 neurology & neurosurgery
Oxidative stress
Zdroj: Journal of Comparative Physiology B-Biochemical systemic and environmental physiology
Journal of Comparative Physiology B-Biochemical systemic and environmental physiology, 2010, 180(2), pp.239-246
ISSN: 1432-136X
0174-1578
Popis: At hatching, breaking eggshell induces a surge in oxygen availability that is likely to generate oxidative stress in newborn chicks. To investigate the involvement of potential adaptive antioxidant mechanisms, we explored some markers of oxidative stress and the regulation of muscle avian uncoupling protein (avUCP) and adenine nucleotide translocase (ANT) in ducklings in the peri-hatching period. When compared with pre-hatching levels, the amount of peroxidized lipids were increased 24 h after external pipping in gastrocnemius muscle (+37%) and heart (+39%) as well as the muscle avUCP mRNA expression (+60%) but the susceptibility of red blood cells to free radicals (a functional test of oxidative status) was not affected. In order to relate these changes to the oxidative transition of hatching, an imposed hypoxia/re-oxygenation protocol was used. Hatched chicks that had spent the last 24 h of incubation in artificial severe hypoxia showed a rise in muscle (+50%) and heart (+69%) lipid peroxidation, an increased susceptibility of red blood cells to free radicals, a marked over-expression of avUCP mRNA (+105%) and a rise in mitochondrial ANT content (+54%). These results suggest that avian UCP and ANT may contribute to prepare incubating eggs to the oxidative stress generated by the hypoxia/re-oxygenation transition naturally occurring at hatching.
Databáze: OpenAIRE
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