The promoter for tomato 3-hydroxy-3-methylglutaryl coenzyme A reductase gene 2 has unusual regulatory elements that direct high-level expression
| Autor: | Jonathon O. Narita, N. D. Daraselia, S. Tarchevskaya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Untranslated region
Transcription Genetic Physiology Molecular Sequence Data Codon Initiator Plant Science Genes Plant Lycopene Solanum lycopersicum Gene Expression Regulation Plant Genes Reporter Translational regulation Gene expression Genetics Amino Acid Sequence RNA Messenger Promoter Regions Genetic Gene Conserved Sequence Messenger RNA biology Base Sequence Sequence Homology Amino Acid food and beverages Promoter biology.organism_classification Carotenoids Cell biology Isoenzymes Biochemistry Regulatory sequence Hydroxymethylglutaryl-CoA-Reductases NADP-dependent RNA Plant Protein Biosynthesis Nucleic Acid Conformation Hydroxymethylglutaryl CoA Reductases Cauliflower mosaic virus Research Article |
| Popis: | The promoter region of tomato (Lycopersicon esculentum) 3-hydroxy-3-methylglutaryl coenzyme A reductase gene 2 (HMG2) has been analyzed using the transient expression of HMG2-luciferase fusions in red fruit pericarp. The mRNA for HMG2 accumulates to high levels during fruit ripening, in a pattern that coincides with the synthesis of the carotenoid lycopene. Unlike most promoters, the region that is upstream of the HMG2 TATA element is not required for high-level expression. The 180-bp region containing the TATA element, the 5[prime] untranslated region, and the translation start site are comparable in strength to the full-length 35S cauliflower mosaic virus promoter. Pyrimidine-rich sequences present in the 5[prime] untranslated leader are important in regulating expression. Also, the ATG start region has been found to increase translation efficiency by a factor of 4 to 10. An alternative hairpin secondary structure has been identified surrounding the HMG2 initiator ATG, which could participate in the translational regulation of this locus. HMG2 appears to be a novel class of strong plant promoters that incorporate unusual, positive regulators of gene expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|