Expression of adipocyte biomarkers in a primary cell culture models reflects preweaning adipobiology
| Autor: | Leslie P. Kozak, Dinh-Toi Chu, Barbara Gawronska-Kozak, Elzbieta Malinowska |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Adipose tissue Gene Expression White adipose tissue Biology Biochemistry Models Biological chemistry.chemical_compound Mice Adipocyte Internal medicine Brown adipose tissue Gene expression medicine Adipocytes Animals Humans Molecular Biology Cells Cultured Adipogenesis Mesenchymal stem cell Cell Biology Mice Inbred C57BL medicine.anatomical_structure Endocrinology Metabolism chemistry Adipose Tissue Cell culture Female Additions and Corrections Biomarkers |
| Popis: | A cohort of genes was selected to characterize the adipogenic phenotype in primary cell cultures from three tissue sources. We compared the quantitative expression of biomarkers in culture relative to their expression in vivo because the mere presence or absence of expression is minimally informative. Although all biomarkers analyzed have biochemical functions in adipocytes, the expression of some of the biomarkers varied enormously in culture relative to their expression in the adult fat tissues in vivo, i.e. inguinal fat for white adipocytes and brite cells, interscapular brown adipose tissue for brown adipocytes, and ear mesenchymal stem cells for white adipocytes from adult mice. We propose that the pattern of expression in vitro does not reflect gene expression in the adult mouse; rather it is predominantly the expression pattern of adipose tissue of the developing mouse between birth and weaning. The variation in gene expression among fat depots in both human and rodent has been an extensively studied phenomenon, and as recently reviewed, it is related to subphenotypes associated with immune function, the inflammatory response, fat depot blood flow, and insulin sensitivity. We suggest that adipose tissue biology in the period from birth to weaning is not just a staging platform for the emergence of adult white fat but that it has properties to serve the unique needs of energy metabolism in the newborn. A case in point is the differentiation of brite cells that occurs during this period followed by their involution immediately following weaning. |
| Databáze: | OpenAIRE |
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