DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain
| Autor: | Gavin S. McNee, Jing Zhang, Durga Pokharel, Grant S. Stewart, Ryan C James, Yongqing Zhang, John J. Reynolds, Michael M. Seidman, Marina A. Bellani, Andrew P. Jackson |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Euchromatin DNA Replication Timing Heterochromatin Science General Physics and Astronomy Cell Cycle Proteins Heterochromatin/metabolism 02 engineering and technology Biology Article Chromosomes General Biochemistry Genetics and Molecular Biology S Phase chemistry.chemical_compound 03 medical and health sciences Gene duplication Humans FANCM lcsh:Science 030304 developmental biology Nuclear Proteins/metabolism 0303 health sciences Replication timing Multidisciplinary Cell Cycle Proteins/metabolism 030302 biochemistry & molecular biology DNA Helicases Nuclear Proteins General Chemistry 021001 nanoscience & nanotechnology DNA Helicases/metabolism Cell biology Chromatin 030104 developmental biology chemistry Chromatin Immunoprecipitation Sequencing Euchromatin/metabolism Replisome lcsh:Q 0210 nano-technology DNA HeLa Cells |
| Zdroj: | Zhang, J, Bellani, M A, James, R C, Pokharel, D, Zhang, Y, Reynolds, J J, McNee, G S, Jackson, A P, Stewart, G S & Seidman, M M 2020, ' DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain ', Nature Communications, vol. 11, no. 1, pp. 3951 . https://doi.org/10.1038/s41467-020-17449-1 Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-17449-1 |
| Popis: | Duplication of mammalian genomes requires replisomes to overcome numerous impediments during passage through open (eu) and condensed (hetero) chromatin. Typically, studies of replication stress characterize mixed populations of challenged and unchallenged replication forks, averaged across S phase, and model a single species of “stressed” replisome. Here, in cells containing potent obstacles to replication, we find two different lesion proximal replisomes. One is bound by the DONSON protein and is more frequent in early S phase, in regions marked by euchromatin. The other interacts with the FANCM DNA translocase, is more prominent in late S phase, and favors heterochromatin. The two forms can also be detected in unstressed cells. ChIP-seq of DNA associated with DONSON or FANCM confirms the bias of the former towards regions that replicate early and the skew of the latter towards regions that replicate late. Eukaryotic replisomes are multiprotein complexes. Here the authors reveal two distinct stressed replisomes, associated with DONSON and FANCM, displaying a bias in replication timing and chromatin domain. |
| Databáze: | OpenAIRE |
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