Evolution of the long range structure of satellite DNAs in the genus Apodemus
| Autor: | H.J. Cooke |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Satellite DNA
Trimer DNA Satellite Biology HindIII Nucleic Acid Denaturation Mice chemistry.chemical_compound Species Specificity Structural Biology Animals Molecular Biology Electrophoresis Agar Gel Genetics Base Sequence Phylogenetic tree Temperature DNA DNA Restriction Enzymes biology.organism_classification Biological Evolution Molecular Weight Restriction enzyme chemistry Agarose gel electrophoresis Nucleic Acid Renaturation biology.protein Satellite (biology) |
| Zdroj: | Journal of Molecular Biology. 94:87-99 |
| ISSN: | 0022-2836 |
| Popis: | DNAs from mice in the genus Apodemus show bands when digests of total DNA with a variety of restriction enzymes are analysed by agarose gel electrophoresis. From the observed patterns a phylogenetic tree can be drawn. In two species, A. sylvaticus and A. flavicollis , the bands with restriction endonuclease from Haemophilus influenzae (Hind III) are integral multiples of a size of 370 base-pairs and derive from satellite DNAs. Divergence in the satellite DNA sequence can be estimated from the proportions of the monomer, dimer and trimer, and from the thermal stability of the DNAs after reassociation. There is a marked difference between the melting behaviour of A. sylvaticus and A. flavicollis DNAs and it is suggested that this is due to the presence of a number of related families of sequences in A. sylvaticus satellite DNA but only one family in A. flavicollis . The observation that these different families of repeated sequences have the same periodicity is consistent with the view that unequal crossing-over is responsible for their evolution into separate families. |
| Databáze: | OpenAIRE |
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