Up-regulation of Nrf2-antioxidant signaling by Açaí (Euterpe oleracea Mart.) extract prevents oxidative stress in human endothelial cells
| Autor: | Mariana Monteiro, Elisa B. Monteiro, Kim Ohanna Pimenta Inada, Nathália Moura-Nunes, Alexandre G. Torres, Elaine Soares, Christophe O. Soulage, Cristiane Maria Amorim Costa, Daniel Perrone, Graziele Freitas de Bem, Angela Castro Resende, Julio Beltrame Daleprane |
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| Přispěvatelé: | State University of Rio de Janeiro, Universidade Federal do Rio de Janeiro [Rio de Janeiro] (UFRJ), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Universidade Federal do Rio de Janeiro (UFRJ), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), FAPERJ [E-26/202.829/2015], [E-26/010.002632/2014], Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [472711/2013-0], CAPES |
| Rok vydání: | 2017 |
| Předmět: |
HPLC-DAD-MS
0301 basic medicine MAPK/ERK pathway animal structures Antioxidant [SDV]Life Sciences [q-bio] medicine.medical_treatment Medicine (miscellaneous) 030204 cardiovascular system & hematology medicine.disease_cause 03 medical and health sciences HUVEC 0302 clinical medicine Downregulation and upregulation medicine TX341-641 Cytotoxicity polyphenols ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Nutrition and Dietetics Nutrition. Foods and food supply Chemistry TEAC Folin-Ciocalteu In vitro 030104 developmental biology Enzyme Biochemistry Polyphenol FRAP Oxidative stress Food Science |
| Zdroj: | Journal of Functional Foods Journal of Functional Foods, Elsevier, 2017, 37, pp.107-115. ⟨10.1016/j.jff.2017.07.035⟩ Journal of Functional Foods, Vol 37, Iss, Pp 107-115 (2017) |
| ISSN: | 1756-4646 |
| Popis: | International audience; The current study evaluated the content and profile of phenolic compounds, the in vitro antioxidant activity, and the role of the Nrf2-signaling pathway in the cellular antioxidant activity of acal seed extract (ASE) in H2O2-induced oxidative stress in human endothelial cells (HUVEC). Seven phenolic compounds were quantified in ASE by HPLC-DAD-MS, among which (-)-epicatechin and (+)-catechin were the most abundant. ASE exhibited a potent in vitro antioxidant activity evaluated by different assays. In HUVEC, ASE decreased H2O2-induced cytotoxicity, prevented migratory capacity loss and oxidative stress. ASE promoted up-regulation of the master antioxidant transcription factor Nrf2 through activation of ERIC, thus leading to an increased expression of antioxidant enzymes. Our results demonstrated that ASE, rich in phenolic compounds, reversed H2O2-induced oxidative stress and that this effect was partially attributed to positive modulation of Nrf2 signaling pathway in HUVEC. |
| Databáze: | OpenAIRE |
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