A single bout of high-intensity exercise modulates the expression of vitamin D receptor and vitamin D-metabolising enzymes in horse skeletal muscle
| Autor: | Chanikarn Puangthong, Patskit Sukhong, Pattrawut Saengnual, Ratchakrit Srikuea, Metha Chanda |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Vitamin
Male medicine.medical_specialty 040301 veterinary sciences Population Calcitriol receptor 0403 veterinary science chemistry.chemical_compound CYP24A1 Internal medicine Vitamin D and neurology Medicine Animals Horses Longitudinal Studies Vitamin D education Muscle Skeletal Vitamin D3 24-Hydroxylase education.field_of_study biology Glycogen business.industry 0402 animal and dairy science Skeletal muscle 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Endocrinology medicine.anatomical_structure chemistry biology.protein Receptors Calcitriol Creatine kinase Female business |
| Zdroj: | Equine veterinary journalREFERENCES. 53(4) |
| ISSN: | 2042-3306 |
| Popis: | Background The expressions of vitamin D receptor (VDR) and vitamin D-metabolising enzymes (CYP27B1 and CYP24A1) in skeletal muscle have been reported. However, the regulation of this vitamin D system in horse skeletal muscle after high-intensity exercise has not yet been elucidated. Objectives To investigate the effect of high-intensity exercise on the expression of vitamin D system-related proteins in horse skeletal muscle and its associations with skeletal muscle stem cell (SMSC) activity and serum 25(OH)D level. Study design Longitudinal study. Methods Six healthy ponies (5 geldings, 1 mare; age 6.3 ± 2.2 years) were studied. Serum and muscle samples were taken from the jugular vein and gluteus medius respectively. Samples were collected at pre-exercise, post-exercise, 1 and 3 weeks after a single bout of high-intensity exercise. Protein expression levels of VDR, CYP27B1, CYP24A1, OxPhos and Pax7 (SMSC marker) were determined using immunohistochemical analysis. Oxidative capacity and intramuscular glycogen content were evaluated using histochemical analysis. Blood biochemistry was analysed for lactate concentration and creatine kinase (CK), and 25(OH)D activity. Results High-intensity exercise significantly upregulated Pax7 and VDR protein expression, which correlated with significantly increased blood lactate and serum CK levels immediately post-exercise. Serum 25(OH)D2 level correlated with CYP27B1 protein expression in skeletal muscle, and it reduced significantly immediately post-exercise and at 1 and 3 weeks post-exercise. However, CYP24A1 protein expression was unchanged throughout study periods. Main limitation The healthy ponies could not represent a fit population of racehorses and eventers. Conclusions The rapid increase in Pax7 and VDR protein expression along with serum CK level after high-intensity exercise demonstrated an association between SMSC activity and activation of the vitamin D system in response to muscle injury in horses. Moreover, a decrease in CYP27B1 protein expression, correlated with a reduction in serum 25(OH)D2 , may indicate a compromised vitamin D metabolism after high-intensity exercise. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text