Histone deacetylase 1 (HDAC-1) required for the normal formation of craniofacial cartilage and pectoral fins of the zebrafish
| Autor: | Gaytri Dubey, Renjitha Pillai, Louise E. Coverdale, C. Cristofre Martin |
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| Rok vydání: | 2004 |
| Předmět: |
Embryo
Nonmammalian Time Factors animal structures Morpholino Mesenchyme Embryonic Development Histone Deacetylase 1 Hindbrain Histone Deacetylases Embryonic Structure medicine Animals Zebrafish In Situ Hybridization biology Homozygote Skull Gene Expression Regulation Developmental Extremities Oligonucleotides Antisense Zebrafish Proteins Embryo Mammalian biology.organism_classification Molecular biology HDAC1 Mutagenesis Insertional Cartilage Histone medicine.anatomical_structure embryonic structures biology.protein Alcian Blue Histone deacetylase Developmental Biology |
| Zdroj: | Developmental Dynamics. 231:647-654 |
| ISSN: | 1097-0177 1058-8388 |
| DOI: | 10.1002/dvdy.20168 |
| Popis: | Histone deacetylases interact with nucleosomes to facilitate the formation of transcriptionally repressed chromatin. In the present study, we show that histone deacetylase 1 (hdac-1) is expressed throughout embryonic development of the zebrafish. The expression of hdac-1 is ubiquitous in early embryos (2-16 hr postfertilization), but at later stages (36 and 48 hr postfertilization), it is primarily restricted to the branchial arches, fin bud mesenchyme, and hindbrain. We report the phenotypes of hdac-1 homozygous mutant embryos and embryos injected with an hdac-1 antisense morpholino. These embryos possess a complex phenotype affecting several embryonic structures. We observed developmental abnormalities in the heart and neural epithelial structures, including the retina and the loss of craniofacial cartilage and pectoral fins. |
| Databáze: | OpenAIRE |
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