The Effects of Resveratrol on Hyperoxia-induced Lung Injury in Neonatal Rats
| Autor: | Ferda Bir, Ersin Gözkeser, Özmert M.A. Özdemir, Cigdem Yenisey |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Pathology
antioxidant antioxidant activity Wistar rat resveratrol Resveratrol medicine.disease_cause Antioxidants chemistry.chemical_compound newborn Fibrosis nonsteroid antiinflammatory agent Stilbenes oxidative stress rat animal glutathione Lung Bronchopulmonary Dysplasia Hyperoxia tumor necrosis factor alpha biology pathogenesis Anti-Inflammatory Agents Non-Steroidal lcsh:RJ1-570 Lung Injury respiratory system superoxide dismutase enzyme activity immunoglobulin enhancer binding protein immunohistochemistry histopathology medicine.symptom Infant Premature medicine.medical_specialty animal experiment Lung injury lung parenchyma Article animal tissue Nitric oxide Superoxide dismutase body weight nitric oxide Internal medicine medicine biochemistry Animals Humans hyperoxia-induced lung injury controlled study human Pediatrics Perinatology and Child Health Rats Wistar nonhuman business.industry animal model disease model lung fibrosis prematurity Infant Newborn lcsh:Pediatrics antiinflammatory activity medicine.disease Disease Models Animal Endocrinology Animals Newborn chemistry Bronchopulmonary dysplasia smooth muscle actin Pediatrics Perinatology and Child Health biology.protein business metabolism Oxidative stress stilbene derivative |
| Zdroj: | Pediatrics and Neonatology, Vol 55, Iss 5, Pp 352-357 (2014) |
| ISSN: | 1875-9572 |
| DOI: | 10.1016/j.pedneo.2013.11.004 |
| Popis: | Background: Bronchopulmonary dysplasia (BPD) is a chronic lung disease that causes significant morbidity and mortality in premature infants. Inflammation and oxidative injury play an important role in the pathogenesis of BPD. Resveratrol is an antioxidant and anti-inflammatory agent. In this study, the histopathological and biochemical effects of resveratrol on a hyperoxia-induced lung injury model in newborn rats were investigated. Methods: The experiment was performed on newborn rat pups from the 3rd to 13th postnatal day and they were randomly divided into four groups: Group 1 (air-exposed + saline, n Z 10), Group 2 (air-exposed + resveratrol, n Z 11), Group 3 (hyperoxia-exposed + saline, n Z 6) and Group 4 (hyperoxia-exposed + resveratrol, n Z 7). Resveratrol was administered (30 mg/kg/day) intraperitoneally. The histopathological effects of resveratrol on lung tissue were assessed by alveolar surface area, fibrosis, and smooth muscle actin (SMA) score, and the biochemical effects on lung tissue were assessed by glutathione (GSH), superoxide dismutase (SOD), nitric oxide (NO), tumor necrosis factor-? (TNF-?), and nuclear factor kappa B (NF-?B) levels. Results: The alveolar surface area, fibrosis, SMA score, and NO levels were found to be significantly higher in Group 3 compared with Group 1 (p < 0.05). In addition, it was found that resveratrol treatment significantly reduced the SMA score and the NO and TNF-? levels, and increased the GSH and SOD levels in the hyperoxia group (p < 0.05). Conclusion: This experimental study showed that oxidative stress and NO contributed to the pathogenesis of hyperoxia-induced lung injury, and that resveratrol had a preventive effect on hyperoxic lung injury through its anti-inflammatory and antioxidant properties. © 2014, Taiwan Pediatric Association. Published by Elsevier Taiwan LLC. All rights reserved. |
| Databáze: | OpenAIRE |
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