Precision cut lung slices: a novel versatile tool to examine host–pathogen interaction in the chicken lung

Autor: Paul Digard, Catherine Schouler, Lonneke Vervelde, Gerry McLachlan, Sascha Trapp, Rodrigo Guabiraba, Karen Bryson, Marco Esposito, Damien Garrido
Přispěvatelé: Division of Infection & Immunity [Roslin, Royaume-Uni], The Roslin Institute [Roslin, Royaume-Uni], University of Edinburgh-University of Edinburgh, Infectiologie et Santé Publique (UMR ISP), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Division of Developmental Biology, the Roslin Institute, University of Edinburgh, ANIHWA (Animal Health and Welfare—ERA-Net), MICHIC project ANR-14-ANWA-0001, Delta-Flu grant agreement No. 727922, BBSRC (BB/M028208/1), USDA-NIFA Agriculture and Food Research Initiative Competitive Grant No. 2015-67015-23093, NARF (BB/J004219/1), BBS/E/D/20002172, BBS/E/D/20002174, Bryson, Karen Jane, Guabiraba, Rodrigo, Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Lipopolysaccharides
Lipopolysaccharide
Virologie
[SDV]Life Sciences [q-bio]
Cell
souche apec
chemistry.chemical_compound
virus influenza aviaire
coupe fine
Lung
Animal biology
lcsh:Veterinary medicine
qrt pcr
Microbiology and Parasitology
Microbiologie et Parasitologie
3. Good health
Cell biology
medicine.anatomical_structure
Host-Pathogen Interactions
Veterinary medicine and animal Health
escherichia coli
réponse inflammatoire
Research Article
Veterinary Medicine
Host–pathogen interaction
030106 microbiology
microscopie confocale
poulet
Biology
03 medical and health sciences
Immune system
In vivo
Live cell imaging
Virology
Biologie animale
medicine
Animals
Poultry Diseases
General Veterinary
interaction hôte pathogène
In vitro
030104 developmental biology
Médecine vétérinaire et santé animal
chemistry
poumon
ex vivo
lcsh:SF600-1100
Chickens
Ex vivo
Zdroj: Veterinary Research
Veterinary Research, BioMed Central, 2020, 51 (1), 16 p. ⟨10.1186/s13567-019-0733-0⟩
Bryson, K, Garrido, D, Esposito, M, McLachlan, G, Digard, P, Schouler, C, Guabiraba, R, Trapp, S & Vervelde, L 2020, ' Precision cut lung slices: a novel versatile tool to examine host-pathogen interaction in the chicken lung ', Veterinary Research, vol. 51, 2 . https://doi.org/10.1186/s13567-019-0733-0
Veterinary Research 1 (51), 16 p.. (2020)
Veterinary Research, Vol 51, Iss 1, Pp 1-16 (2020)
ISSN: 0928-4249
1297-9716
DOI: 10.1186/s13567-019-0733-0⟩
Popis: The avian respiratory tract is a common entry route for many pathogens and an important delivery route for vaccination in the poultry industry. Immune responses in the avian lung have mostly been studied in vivo due to the lack of robust, relevant in vitro and ex vivo models mimicking the microenvironment. Precision-cut lung slices (PCLS) have the major advantages of maintaining the 3-dimensional architecture of the lung and includes heterogeneous cell populations. PCLS have been obtained from a number of mammalian species and from chicken embryos. However, as the embryonic lung is physiologically undifferentiated and immunologically immature, it is less suitable to examine complex host–pathogen interactions including antimicrobial responses. Here we prepared PCLS from immunologically mature chicken lungs, tested different culture conditions, and found that serum supplementation has a detrimental effect on the quality of PCLS. Viable cells in PCLS remained present for ≥ 40 days, as determined by viability assays and sustained motility of fluorescent mononuclear phagocytic cells. The PCLS were responsive to lipopolysaccharide stimulation, which induced the release of nitric oxide, IL-1β, type I interferons and IL-10. Mononuclear phagocytes within the tissue maintained phagocytic activity, with live cell imaging capturing interactions with latex beads and an avian pathogenic Escherichia coli strain. Finally, the PCLS were also shown to be permissive to infection with low pathogenic avian influenza viruses. Taken together, immunologically mature chicken PCLS provide a suitable model to simulate live organ responsiveness and cell dynamics, which can be readily exploited to examine host–pathogen interactions and inflammatory responses.
Databáze: OpenAIRE
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