Circulating serum CK level vs. muscle impairment for in situ monitoring burden of disease in Mdx-mice
| Autor: | Katharina Zeitler, J. Dolderer, Jody Vykoukal, Lukas Prantl, Oliver Felthaus, Silvan Klein, Sebastian Geis, Eckhard Alt, Alexandra Anker |
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| Rok vydání: | 2017 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Burden of disease In situ medicine.medical_specialty mdx mouse Physiology Duchenne muscular dystrophy Dystrophin Mice 03 medical and health sciences 0302 clinical medicine Muscular Diseases Physiology (medical) Internal medicine medicine Animals Creatine Kinase biology business.industry Hematology medicine.disease Phenotype Mice Inbred C57BL Muscular Dystrophy Duchenne Disease Models Animal 030104 developmental biology Endocrinology Muscle dysfunction Mice Inbred mdx biology.protein Female Creatine kinase Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery |
| Zdroj: | Clinical Hemorheology and Microcirculation. 65:327-334 |
| ISSN: | 1875-8622 1386-0291 |
| DOI: | 10.3233/ch-16195 |
| Popis: | Background Duchenne muscular dystrophy (DMD) consists of a lack in the expression of the subsarcolemmal protein dystrophin causing progressive muscle dysfunction. Among the widely applied animal models in DMD research is the C57BL/1010ScSn-Dmdmdx mouse, commonly referred to as the "mdx mouse". The potential benefit of novel interventions in this model is often assessed by variables such as functional improvement, histological changes, and creatine kinase (CK) serum levels as an indicator for the extent of in situ muscle damage. Objective Our objective was to determine to what extent the serum CK-level serves a surrogate for muscle dysfunction. Methods In this trial mdx mice were subjected to a four-limb wire-hanging test (WHT) to assess the physical performance as a reference for muscle function. As CK is a component of the muscle fiber cytosol, its serum activity is supposed to positively correlate with progressing muscle damage. Hence serum CK levels were measured to detect the degree of muscle impairment. The functional tests and the serum CK levels were analyzed for their specific correlation. Results Although physical performance decreased during the course of the experiment, latency to fall times in the WHT did not correlate with the CK level in mdx mice. Conclusion Our data suggests that the serum CK activity might be a critical parameter to monitor the progression of muscle impairment in mdx mice. Further this study emphasizes the complexity of the DMD phenotype in the mdx mouse, and the care with which isolated parameters in this model should be interpreted. |
| Databáze: | OpenAIRE |
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