Which Form of Dopamine Is the Substrate for the Human Dopamine Transporter: the Cationic or the Uncharged Species?
| Autor: | Lijuan C. Wang, Maarten E.A. Reith, Janet L. Berfield |
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| Rok vydání: | 1999 |
| Předmět: |
Guanethidine
Stereochemistry Dopamine Dopamine Plasma Membrane Transport Proteins Nerve Tissue Proteins Biochemistry Cell Line chemistry.chemical_compound Cocaine Dopamine Uptake Inhibitors medicine Humans Binding site Molecular Biology Dopamine transporter Neurons Dopamine binding Binding Sites Membrane Glycoproteins biology Chemistry Bretylium Compounds Membrane Transport Proteins Tropane Transporter Cell Biology Hydrogen-Ion Concentration biology.protein Carrier Proteins medicine.drug |
| Zdroj: | Journal of Biological Chemistry. 274:4876-4882 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.274.8.4876 |
| Popis: | The question of which is the active form of dopamine for the neuronal dopamine transporter is addressed in HEK-293 cells expressing the human dopamine transporter. The Km value for [3H]dopamine uptake fell sharply when the pH was increased from 6.0 to 7.4 and then changed less between pH 7.4 and 8.2. The KI for dopamine in inhibiting the cocaine analog [3H]2beta-carbomethoxy-3beta-(4-fluorophenyl)tropane binding displayed an identical pH dependence, suggesting that changes in uptake result from changes in dopamine recognition. Dopamine can exist in the anionic, neutral, cationic, or zwitterionic form, and the contribution of each form was calculated. The contribution of the anion is extremely low ( |
| Databáze: | OpenAIRE |
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