Conjugated Linoleic Acid Reduces Phorbol Ester-Induced Prostaglandin F2 Production by Bovine Endometrial Cells
| Autor: | Lokenga Badinga, C. Rodriguez-Sallaberry, E.S. Greene, Cristina Caldari-Torres |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Conjugated linoleic acid Linoleic acid Biology Dinoprost Endometrium chemistry.chemical_compound Internal medicine Genetics medicine Animals Linoleic Acids Conjugated Secretion PPAR delta Receptor Cells Cultured Phorbol 12 13-Dibutyrate DNA Primers Messenger RNA food and beverages Peroxisome Endocrinology Prostaglandin F2alpha chemistry Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Phorbol Cattle Female lipids (amino acids peptides and proteins) Animal Science and Zoology Food Science medicine.drug |
| Zdroj: | Journal of Dairy Science. 89:3826-3832 |
| ISSN: | 0022-0302 |
| Popis: | Recent interest in conjugated linoleic acid (CLA) research stems from the well-documented anticarcinogenic, antiatherogenic, antidiabetic, and antiobesity properties of CLA in animal models. The objective of this study was to examine the effects of 2 CLA isomers (cis-9,trans-11 and trans-10,cis-12) on phorbol 12,13-dibutyrate (PDBu)-induced PGF2alpha production in cultured bovine endometrial (BEND) cells. Confluent BEND cells were incubated in the absence (control) or presence of 100 microM each of linoleic acid, cis-9,trans-11 CLA, or trans-10,cis-12 CLA for 24 h. After incubation, cells were rinsed and then stimulated with PDBu (100 ng/mL) for 6 h. Compared with untreated cells, PDBu stimulated PGF2alpha secretion (+25-fold) within 6 h. The increases in PGF(2alpha) secretion were paralleled by signifi-cant induction of prostaglandin endoperoxide synthase-2 (PGHS-2) mRNA (+63-fold) and protein (+1.6-fold) expression. In spite of stimulatory effects on PGHS-2 and peroxisome proliferator-activated receptor delta (PPARdelta) mRNA responses, CLA greatly decreased PGF2alpha production by PDBu-stimulated BEND cells. There was no evidence for PDBu or CLA modulation of PPARdelta protein synthesis in cultured BEND cells. Results indicated that CLA modulation of PGF2alpha production by BEND cells was not mediated through PGHS-2 or PPARdelta gene repression. |
| Databáze: | OpenAIRE |
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