Development of a novel and rapid measurement system for growth differentiation factor-15, progranulin, and osteopontin in uterine sarcoma
| Autor: | Yoshio Yoshida, Hideaki Tsuyoshi, Tetsuya Mizutani, Kaoru Miyamoto, Shinya Kawabe, Maeno Mitsuo, Masami Yamada, Shizuka Yamada, Kengo Nishimura, Shin Ishikane |
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| Rok vydání: | 2019 |
| Předmět: |
Poor prognosis
Diagnostic methods Growth Differentiation Factor 15 Time Factors Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism Pilot Projects Sensitivity and Specificity Diagnosis Differential 03 medical and health sciences 0302 clinical medicine Endocrinology Progranulins medicine Humans Osteopontin Immunoassay medicine.diagnostic_test Uterine sarcoma biology Leiomyoma business.industry Benign Uterine Tumors Sarcoma Serum concentration medicine.disease ROC Curve 030220 oncology & carcinogenesis Uterine Neoplasms Cancer research biology.protein Female GDF15 business |
| Zdroj: | Endocrine journal. 67(1) |
| ISSN: | 1348-4540 |
| Popis: | Uterine sarcomas are rare and aggressive gynecologic tumors with poor prognosis; therefore, early diagnosis is crucial for therapy. However, it is very difficult to distinguish uterine sarcomas from leiomyomas which are common benign uterine tumors. Therefore, the development of a diagnostic method that utilizes reliable biomarkers to distinguish uterine sarcomas from leiomyomas is important so as to identify the rare tumors. The candidate factors as novel biomarkers were searched for in public databases and a pilot study was performed for confirmation. Growth differentiation factor-15 (GDF15), progranulin, and osteopontin were identified as candidate biomarkers for diagnosing uterine sarcoma. Thus, developing a rapid and easy method to measure these factors could help establish a screening system for uterine sarcomas. In this study, we developed a novel measurement system for these factors using a compact chemical luminescence immunological automatic analyzer POCubeTM. This assay system, which is based on the flow-through membrane immunoassay, completes the whole process and generates results within 15 min. Serum concentrations of these factors measured via POCubeTM correlated well with those measured using enzyme-linked immunosorbent assay (r = 0.994 for GDF15, r = 0.992 for progranulin, and r = 0.976 for osteopontin). The POCubeTM system provides rapid and easy measurement of these factors, thereby facilitating uterine sarcoma diagnosis. |
| Databáze: | OpenAIRE |
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