MiR-17-5p and miR-20a promote chicken cell proliferation at least in part by upregulation of c-Myc via MAP3K2 targeting
| Autor: | Xiaohong Yan, Shupei Qiao, He Song, Hui Li, Jing Liu, Xiaofei Zhang, Tianyu Xing, Ning Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Apoptosis MAP Kinase Kinase Kinase 2 MAP3K2 Article Proto-Oncogene Proteins c-myc 03 medical and health sciences Downregulation and upregulation Cell Movement RNA interference Gene expression Animals Humans E2F1 lcsh:Science Cell Proliferation Gene knockdown Multidisciplinary Cell growth Mir 17 5p Chemistry lcsh:R Cell biology MicroRNAs 030104 developmental biology Gene Expression Regulation RNA Interference lcsh:Q Chickens E2F1 Transcription Factor Signal Transduction |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-16 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The miR-17-92 cluster has been well studied in mammals but less extensively studied in birds. Here, we demonstrated that miR-17-92 cluster overexpression promoted the proliferation of DF1 cells and immortalized chicken preadipocytes (ICPA-1), and miR-17-5p and miR-20a, members of the miR-17-92 cluster, targeted MAP3K2. Further analysis showed that MAP3K2 overexpression reduced the proliferation of DF1 and ICPA-1 cells and attenuated the promotive effect of the miR-17-92 cluster on cell proliferation. Downstream gene expression analysis of the MAPK signalling pathway showed that MAP3K2 overexpression decreased c-Myc expression; in contrast, MAP3K2 knockdown using RNA interference and miR-17-92 cluster overexpression increased c-Myc expression. Furthermore, c-Myc overexpression promoted miR-17-92 cluster expression and DF1 cell proliferation. Taken together, these data indicated that miR-17-92 promotes chicken cell proliferation at least in part by the upregulation of c-Myc via targeting MAP3K2, and the miR-17-92 cluster, c-Myc and E2F1 form a complex regulatory network in chicken cell proliferation. |
| Databáze: | OpenAIRE |
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