Sperm chromatin structure assay versus sperm chromatin dispersion kits: Technical repeatability and choice of assisted reproductive technology procedure
| Autor: | Ramyashree Devaraj, Sridurga Mithraprabhu, Tara Mahendran, Silviya Stephen, Vidya Laxme B, Ricardo Pimenta Bertolla |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Infertility Male infertility 030219 obstetrics & reproductive medicine Assisted reproductive technology In vitro fertilisation business.industry medicine.medical_treatment DNA fragmentation Repeatability medicine.disease Sperm Intracytoplasmic sperm injection Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Reproductive Medicine medicine Original Article business |
| Zdroj: | Clinical and Experimental Reproductive Medicine |
| ISSN: | 2233-8241 2233-8233 |
| DOI: | 10.5653/cerm.2020.03860 |
| Popis: | Objective: The sperm DNA fragmentation index (DFI) guides the clinician’s choice of an appropriate assisted reproductive technology (ART) procedure. The DFI can be determined using commercially available methodologies, including sperm chromatin dispersion (SCD) kits and sperm chromatin structure assay (SCSA). Currently, when DFI is evaluated using SCD kits, the result is analyzed in reference to the SCSA-derived threshold for the choice of an ART procedure. In this study, we compared DFI values obtained using SCSA with those obtained using SCD and determined whether the difference affects the choice of ART procedure.Methods: We compared SCSA to two SCD kits, CANfrag (n=36) and Halosperm (n=31), to assess the DFI values obtained, the correlations between tests, the technical repeatability, and the impact of DFI on the choice of ART. Results: We obtained higher median DFI values using SCD kits than when using SCSA, and this difference was significant for the CANfrag kit (p |
| Databáze: | OpenAIRE |
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