Effect of Resveratrol on Bcl-2 and VEGF Expression in Oxygen-Induced Retinopathy of Prematurity
| Autor: | Wenlin Li, Deyong Jiang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Blotting Western Enzyme-Linked Immunosorbent Assay Resveratrol Group A Antioxidants Rats Sprague-Dawley chemistry.chemical_compound Western blot Internal medicine Stilbenes medicine Animals Humans Retinopathy of Prematurity Oxygen toxicity Dose-Response Relationship Drug medicine.diagnostic_test business.industry Infant Newborn Retinopathy of prematurity General Medicine medicine.disease Rats Oxygen Blot Vascular endothelial growth factor Disease Models Animal Ophthalmology Dose–response relationship Endocrinology Animals Newborn Proto-Oncogene Proteins c-bcl-2 chemistry Pediatrics Perinatology and Child Health business Platelet Aggregation Inhibitors |
| Zdroj: | Journal of Pediatric Ophthalmology & Strabismus. 49:230-235 |
| ISSN: | 1938-2405 0191-3913 |
| DOI: | 10.3928/01913913-20111129-01 |
| Popis: | Purpose: To explore the effect of resveratrol on B-cell leukemia/lymphoma-2 (Bcl-2) and vascular endothelial growth factor (VEGF) expression in rats with oxygen-induced retinopathy of prematurity (ROP). Methods: Seven-day-old Sprague-Dawley rats (N = 60) were randomly assigned to five groups. Group A received normal partial oxygen pressure and groups B, C, D, and E received 75% ± 2% oxygen for 5 days to induce ROP. The rats in groups C, D, and E were intragastrically treated with resveratrol (10, 30, and 60 mg/kg/d, respectively) once daily for 5 days. Rats were killed at 17 days of age and the retina was collected. Results: Western blot analysis revealed increased Bcl-2 protein expression in group B versus group A. Levels of Bcl-2 decreased with the increase of resveratrol concentration in groups C, D, and E. The optical density of Bcl-2 protein expression in group B was four times higher than that in group A ( P < .01). When compared with group B, expression of Bcl-2 and VEGF in groups C, D, and E decreased in a dose-dependent manner. Significant differences in expression of Bcl-2 and VEGF were also noted among the three treatment groups with resveratrol ( P < .01). After treatment with resveratrol at 10, 30, and 60 mg/kg/d, the inhibition rate of Bcl-2 expression was 11.1%, 38.1%, and 69.8% and that of VEGF expression was 3.4%, 23.0%, and 43.7%, respectively. Conclusion: Resveratrol can significantly inhibit expression of Bcl-2 and VEGF in the retina of neonatal rats with oxygen-induced ROP. It may provide a protective effect on retinal neovascular diseases, including ROP. |
| Databáze: | OpenAIRE |
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