Ex Vivo Hemodialysis Culture of Microbial and Mammalian Cells
| Autor: | Philipp Gerhardt, Ralph C. Belding, John M. Quarles, T C Beaman |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Erythrocytes
Time Factors medicine.medical_treatment Microorganism Tissue membrane Biology Kidney Cell Line Microbiology Mice Dialysis solutions Arteriovenous Shunt Surgical Renal Dialysis Cricetinae Mammalian cell Candida albicans Leukocytes medicine Animals Humans Immunology and Allergy Anaerobiosis Maximal rate Cells Cultured Serratia marcescens Bacteriological Techniques Goats Obligate anaerobe Membranes Artificial Kinetics Infectious Diseases Immunology Hemodialysis Kidneys Artificial Spleen Ex vivo |
| Zdroj: | Journal of Infectious Diseases. 135:42-50 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/135.1.42 |
| Popis: | A small hemodialysis culture unit was developed which can be attached to an arterial-venous shunt and worn by an animal for days. The unit consists of a blood channel separated by a membrane from a dialysate chamber in which microbial or mammalian cells can be cultured. Bacterial multiplication proceeded first exponentially at the maximal rate and then arithmetically at a lesser, dialysis-limited rate. In a survey of 16 pathogenic microorganisms and five types of mammalian cell, results indicated that most of the aerobes grew well, but none of the obligate anaerobes grew at all. The separation of the culture from cellular and macromolecular host defense mechanisms allowed the cultivation of parasitic cells on an animal that was not naturally susceptible. |
| Databáze: | OpenAIRE |
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