Effects of methylthiouracil on the proliferation and apoptosis of rat bone marrow stromal cells
| Autor: | Chuan Tian, Jie Ding, Xiao‑Xiao Hou, Ming‑Zhen Chen, Zhong‑Lu Ye, Re‑Ling Chen, Guo‑Hua Zheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Stromal cell proliferation Cell Methylthiouracil Flow cytometry Andrology Immunology and Microbiology (miscellaneous) stomatognathic system Cytotoxic T cell Medicine medicine.diagnostic_test business.industry Cell growth apoptosis methylthiouracil General Medicine Articles Cell cycle medicine.anatomical_structure Apoptosis business medicine.drug bone marrow stromal cells |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | The aim of the present study was to investigate the effects of methylthiouracil (MTU) on the proliferation and apoptosis of rat bone marrow stromal cells (BMSCs). Rat BMSCs were isolated, cultured in vitro and treated with various concentrations of MTU. Cell growth curves were determined using the Cell Counting Kit-8 method and the effect of MTU on BMSCs in a logarithmic growth phase was observed. BMSC apoptosis following MTU treatment was detected by flow cytometry. The experimental results demonstrated that the proliferation-inhibition effect was gradually enhanced with increasing MTU concentrations and the extension of treatment time. Statistically significant differences were observed between the treatment and the control groups (P |
| Databáze: | OpenAIRE |
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