Extraction of Functional Binding Sites from Unique Regulatory Regions: The Drosophila Early Developmental Enhancers
| Autor: | Mireille Régnier, Anna G. Nazina, Vsevolod J. Makeev, Claude Desplan, Dmitri Papatsenko, Alex P. Lifanov |
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| Rok vydání: | 2002 |
| Předmět: |
In silico
Molecular Sequence Data Protozoan Proteins Genes Insect Computational biology Regulatory Sequences Nucleic Acid DNA sequencing Bacterial Proteins Methods Genetics Animals Drosophila Proteins Binding site Enhancer Transcription factor Genetics (clinical) Homeodomain Proteins Binding Sites Base Sequence biology Chromosome Mapping Gene Expression Regulation Developmental Nuclear Proteins biology.organism_classification DNA-Binding Proteins Drosophila melanogaster Enhancer Elements Genetic Tandem Repeat Sequences Regulatory sequence Multigene Family Algorithms Drosophila Protein Transcription Factors |
| Zdroj: | Genome Research. 12:470-481 |
| ISSN: | 1549-5469 1088-9051 |
| DOI: | 10.1101/gr.212502 |
| Popis: | The early developmental enhancers of Drosophila melanogaster comprise one of the most sophisticated regulatory systems in higher eukaryotes. An elaborate code in their DNA sequence translates both maternal and early embryonic regulatory signals into spatial distribution of transcription factors. One of the most striking features of this code is the redundancy of binding sites for these transcription factors (BSTF). Using this redundancy, we explored the possibility of predicting functional binding sites in a single enhancer region without any prior consensus/matrix description or evolutionary sequence comparisons. We developed a conceptually simple algorithm,Scanseq, that employs an original statistical evaluation for identifying the most redundant motifs and locates the position of potential BSTF in a given regulatory region. To estimate the biological relevance of our predictions, we built thorough literature-based annotations for the best-known Drosophiladevelopmental enhancers and we generated detailed distribution maps for the most robust binding sites. The high statistical correlation between the location of BSTF in these experiment-based maps and the location predicted in silico by Scanseqconfirmed the relevance of our approach. We also discuss the definition of true binding sites and the possible biological principles that govern patterning of regulatory regions and the distribution of transcriptional signals. |
| Databáze: | OpenAIRE |
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