Effects of a diphtheria-tetanus-acellular pertussis vaccine on immune responses in murine local lymph node and lung allergy models
| Autor: | Michiel van Hartskamp, Frits R. Mooi, Eric R. Gremmer, Jan A. M. A. Dormans, Rob J. Vandebriel |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Male Bordetella pertussis medicine.medical_treatment Clinical Biochemistry Immunology Immunoglobulin E Diphtheria-Tetanus-acellular Pertussis Vaccines Mice Immune system Species Specificity Transforming Growth Factor beta medicine Hypersensitivity Immunology and Allergy Animals Lung Mice Inbred BALB C biology medicine.diagnostic_test business.industry Local lymph node assay Vaccination respiratory system Local Lymph Node Assay biology.organism_classification Vaccine Research respiratory tract diseases Mice Inbred C57BL Bronchoalveolar lavage Phthalic Anhydrides biology.protein Female Lymph business Adjuvant |
| Zdroj: | Clinical and vaccine immunology : CVI. 14(3) |
| ISSN: | 1556-6811 |
| Popis: | We have previously shown that in mice, diphtheria-tetanus-acellular pertussis (DTaP) vaccination before Bordetella pertussis infection resulted in, besides effective clearance, immediate hypersensitivity (lung eosinophilia, increased total serum immunoglobulin E [IgE], and increased ex vivo Th2 cytokine production by cells from the bronchial lymph nodes). To better appreciate the extent of these findings, we measured DTaP vaccination effects in the local lymph node assay (LLNA) and an ovalbumin (OVA) lung allergy model. In the LLNA, mice were vaccinated or adjuvant treated before being sensitized with trimellitic anhydride (TMA; inducing a Th2-directed response) and dinitrochlorobenzene (DNCB; inducing a Th1-directed response). Compared to the adjuvant-treated controls, the vaccinated mice showed a decreased response to TMA and (to a much lesser extent) an increased response to DNCB. The decreased response to TMA coincided with increased transforming growth factor β levels. With the exception of filamentous hemagglutinin, all vaccine constituents contributed to the decreased response to TMA. In the lung allergy model, sensitization induced OVA-specific IgE, lung pathology (peribronchiolitis, perivasculitis, and hypertrophy of the bronchiolar mucus cells) and increased the number of eosinophils, lymphocytes, and neutrophils in the bronchoalveolar lavage fluid. Vaccination failed to modulate these parameters. In conclusion, although DTaP vaccination may affect the LLNA response, we found no evidence of an effect on lung allergy. |
| Databáze: | OpenAIRE |
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