Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
| Autor: | Maria Silvia Viccari Gatti, Maria Lucia Rácz, Jéssica Wildgrube Bertol, Veridiana Munford, Maria Clara Duarte Fregolente, Marco Aurélio Palazzi Sáfadi, T. A. R. Caruzo, Márcio José da Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Rotavirus Microbiology (medical) lcsh:Arctic medicine. Tropical medicine Genotype Genetic Linkage lcsh:RC955-962 viruses Molecular Sequence Data Reassortment lcsh:QR1-502 Viral Nonstructural Proteins Biology medicine.disease_cause lcsh:Microbiology Immunoenzyme Techniques Feces Genetic linkage NSP4 genotypes Genetic variation human rotavirus medicine Humans Amino Acid Sequence Child Antigens Viral Gene Phylogeny Glycoproteins Toxins Biological Genetics Base Sequence Phylogenetic tree Reverse Transcriptase Polymerase Chain Reaction phylogenetic analysis Nucleic acid sequence Genetic Variation Articles biochemical phenomena metabolism and nutrition Virology RNA Viral VP4/VP7/NSP4 linkage Sequence Alignment Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 110, Issue: 6, Pages: 786-792, Published: SEP 2015 Memórias do Instituto Oswaldo Cruz., Vol 110, Iss 6, Pp 786-792 (2015) Memórias do Instituto Oswaldo Cruz |
| Popis: | Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregation. |
| Databáze: | OpenAIRE |
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