Subcellular Distribution of Enzymes of Glycolate Metabolism in the Alga Cyanidium caldarium

Autor:	Wolfgang Gross, Harry Beevers
Rok vydání:	1989
Předmět:	chemistry.chemical_classification biology Physiology Hydroxypyruvate reductase Plant Science Peroxisome Serine Enzyme Biochemistry chemistry Catalase Serine hydroxymethyltransferase Organelle Genetics biology.protein Microbody Metabolism and Enzymology
Zdroj:	Plant Physiology. 90:799-805
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.90.3.799
Popis:	The intracellular distribution of enzymes capable of catalyzing the reactions from phosphoglycolate to glycerate in the bluegreen colored eucaryotic alga Cyanidium caldarium has been studied. After separating the organelles from a crude homogenate on a linear flotation gradient, the enzymes glycolate oxidase and glutamate-glyoxylate aminotransferase along with catalase were present in the peroxisomal fraction (density: 1.23 grams per cubic centimeter). Serine hydroxymethyltransferase was found in the mitochondrial fraction (density: 1.18 grams per cubic centimeter). In contrast to the observations in green leaves of higher plants, the enzymes for the conversion of serine to glycerate (serine-glyoxylate aminotransferase and hydroxypyruvate reductase) were found only in the soluble fraction of the gradient. The partial characterization of enzymes from Cyanidium participating in glycolate metabolism revealed only slight differences from the corresponding enzymes from higher plants. The phylogenetic implications of the observed similarities between the enigmatic alga Cyanidium and higher plants are discussed.
Databáze:	OpenAIRE
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