Anti-inflammatory activity of low molecular weight polysialic acid on human macrophages
| Autor: | Anahita Shahraz, Jens Kopatz, Dominic Winter, Vlad Schütza, Harald Neumann, Thomas Scheper, Shoba Kapoor, Joachim Kappler, Volkmar Gieselmann, Rene Mathy |
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| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie Lipopolysaccharide Phagocytosis Neuroimmunology Anti-Inflammatory Agents Glycobiology Biology Endocytosis Article Cell Line Polymerization chemistry.chemical_compound Lectins medicine Homeostasis Humans Chromatography High Pressure Liquid Respiratory Burst Amyloid beta-Peptides Multidisciplinary Microglia Tumor Necrosis Factor-alpha Polysialic acid Macrophages Membrane Proteins Molecular biology Microspheres Respiratory burst Sialic acid Molecular Weight Neuroprotective Agents medicine.anatomical_structure chemistry ddc:540 Sialic Acids Tumor necrosis factor alpha |
| Zdroj: | Scientific Reports 5 (2015) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep16800 |
| Popis: | Oligosialic and polysialic acid (oligoSia and polySia) of the glycocalyx of neural and immune cells are linear chains, in which the sialic acid monomers are α2.8-glycosidically linked. Sialic acid-binding immunoglobulin-like lectin-11 (SIGLEC-11) is a primate-lineage specific receptor of human tissue macrophages and microglia that binds to α2.8-linked oligoSia. Here, we show that soluble low molecular weight polySia with an average degree of polymerization 20 (avDP20) interacts with SIGLEC-11 and acts anti-inflammatory on human THP1 macrophages involving the SIGLEC-11 receptor. Soluble polySia avDP20 inhibited the lipopolysaccharide (LPS)-induced gene transcription and protein expression of tumor necrosis factor-α (Tumor Necrosis Factor Superfamily Member 2, TNFSF2). In addition, polySia avDP20 neutralized the LPS-triggered increase in macrophage phagocytosis, but did not affect basal phagocytosis or endocytosis. Moreover, polySia avDP20 prevented the oxidative burst of human macrophages triggered by neural debris or fibrillary amyloid-β1–42. In a human macrophage-neuron co-culture system, polySia avDP20 also reduced loss of neurites triggered by fibrillary amyloid-β1–42. Thus, treatment with polySia avDP20 might be a new anti-inflammatory therapeutic strategy that also prevents the oxidative burst of macrophages. |
| Databáze: | OpenAIRE |
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