Implementation of a novel ultra fast metabolic stability analysis method using exact mass TOF-MS
| Autor: | Joseph D Manna, Samantha J. Richardson, Mehran F. Moghaddam |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Computer science
Chemistry Pharmaceutical Automated data processing Clinical Biochemistry Analytical chemistry 030226 pharmacology & pharmacy 01 natural sciences Stability (probability) Mass Spectrometry Workflow Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Software Tandem Mass Spectrometry Drug Discovery Animals Humans Technology Pharmaceutical General Pharmacology Toxicology and Pharmaceutics Process engineering Chromatography High Pressure Liquid Electronic Data Processing business.industry 010401 analytical chemistry Reproducibility of Results General Medicine Metabolic stability Automation Rats 0104 chemical sciences Molecular Weight Mass Medical Laboratory Technology Liver Time-of-flight mass spectrometry business |
| Zdroj: | Bioanalysis. 9:359-368 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio-2016-0187 |
| Popis: | Aim: Increasing numbers of compounds requiring stability data means highly optimized methods capable of rapid turnaround are desirable during early discovery. Materials and methods/results: An advanced, generic analytical workflow for metabolic stability has been developed that utilizing ballistic gradient LC (sub 1 min run times), exact mass TOF-MS (Waters Xevo-G2-XS Q-TOF) and automated data processing (Waters UNIFI software) allowed for rapid integration and interpretation of all data produced, eliminating the need for method development and manual processing. We can analyze and process 96 compounds across two species in quadruplicate in a 24-h period with no method development. Conclusion: An advanced bioanalytical workflow has increased our capacity threefold and reduced our instrument/processing needs threefold. |
| Databáze: | OpenAIRE |
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