Induction of Apoptosis in SKOV3 and DNA Binding by Cobalt(Iii) Polypyridyl Complexes
| Autor: | M. Ramchander, Ravi Kumar Vuradi, Ch. Ravi, Sirasani Satyanarayana, Srishailam Avudoddi |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
education.field_of_study 010405 organic chemistry Stereochemistry Phenanthroline Organic Chemistry Intercalation (chemistry) Population General Medicine 01 natural sciences Biochemistry 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Apoptosis MTT assay Growth inhibition Cytotoxicity education DNA |
| Zdroj: | Биоорганическая химия. 45:403-403 |
| ISSN: | 0132-3423 |
| Popis: | Three new Co(III) polypyridyl complexes [Co(phen)2CIIP]3+ {CIIP = 2-(5-chloro-3a,H-isoindol-3-yl)-1H-imidazo[4,5-f][1,10]phenantholine} (phen = 1,10 phenanthroline), [Co(bpy)2CIIP]3+ (bpy = 2,2′-bipyridine), and [Co(dmb)2CIIP]3+ (dmb = 4,4′-dimethyl-2,2′-bipyridine) were synthesized and characterized by different spectral methods. The complexes interact with DNA in an intercalation mode as confirmed by spectroscopic titration and viscosity measurements. All three complexes cleaved the pBR322 DNA in photoactivated cleavage studies and exhibited good antimicrobial activity. Anticancer activity of these Co(III) complexes was evaluated on the SKOV3 cell line. Cytotoxicity by MTT assay showed growth inhibition in dose dependent manner. Cell cycle analysis by flow cytometry data showed increase in Sub G1 population. Annexin V FITC/PI staining confirmed that these complexes caused cell death by the induction of apoptosis. |
| Databáze: | OpenAIRE |
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