Purification of Large Quantities of Influenza Virus by Density Gradient Centrifugation
| Autor: | N. G. Anderson, R S Baker, C B Reimer, G. B. Cline, T E Newlin, R. M. vanFrank |
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| Jazyk: | angličtina |
| Rok vydání: | 1967 |
| Předmět: |
Virus Cultivation
Density gradient Immunology Orthomyxoviridae Chick Embryo Microbiology Mice Virology Culture Techniques Animal Viruses Centrifugation Density Gradient Methods Animals Centrifugation Hemagglutination Viral Differential centrifugation Chromatography biology Elution biology.organism_classification Orders of magnitude (mass) Isopycnic Influenza Vaccines Insect Science Rabbits Absorption (chemistry) Barium Sulfate |
| Popis: | New zonal centrifuges can conveniently process as much as five orders of magnitude (10 5 ) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorption and elution of influenza virus with barium sulfate followed by concentration and isopycnic banding of the virus in a buffered sucrose gradient. Kilogram quantities of impurity have been rapidly separated from grams of purified virus, which have been conveniently concentrated several hundred-fold by the purification process. Experimental vaccines made by these procedures are being evaluated. |
| Databáze: | OpenAIRE |
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