Monocytes of Different Subsets in Complexes with Platelets in Patients with Myocardial Infarction
| Autor: | Anush Arakelyan, Leonid Margolis, N. Pinegina, Marina Loguinova, Murad Vagida, Valeria Kogan, Elena Vasilieva, Alexander Shpektor |
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| Rok vydání: | 2018 |
| Předmět: |
Blood Platelets
Male 0301 basic medicine Platelet Aggregation CD14 Lipopolysaccharide Receptors Myocardial Infarction Cell Communication 030204 cardiovascular system & hematology CD16 GPI-Linked Proteins Monocytes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Antigen Antigens CD immune system diseases Annexin Humans Medicine Platelet cardiovascular diseases Platelet activation Receptors Immunologic Cells Cultured Aged business.industry Monocyte Hematology Phosphatidylserine Middle Aged Flow Cytometry 030104 developmental biology medicine.anatomical_structure Platelet Glycoprotein GPIb-IX Complex chemistry Immunology Female business |
| Zdroj: | Thrombosis and Haemostasis. 118:1969-1981 |
| ISSN: | 2567-689X 0340-6245 |
| Popis: | Acute myocardial infarction (AMI) is associated with activation of various cells, including platelets that form monocyte–platelet complexes (MPCs). Here, we analysed MPC in vivo and in vitro and investigated the abilities of different monocyte subclasses to form MPC, the characteristics of the cells involved in MPC formation and MPC changes in AMI. We identified MPC by co-staining for platelet antigen CD41a and monocyte antigens CD14 and CD16. Platelet activation was evaluated from expression of phosphatidylserine as revealed by annexin V. Our results confirm published data and provide new information regarding the patterns of MPC in AMI patients. We found that the patterns of platelet aggregation with monocytes were different in AMI patients and controls: (1) in AMI patients, MPC formed by intermediate monocytes carry more platelets whereas in healthy controls more platelets aggregated with classical monocytes; (2) the numbers of MPC in AMI patients, being already higher than in controls, were further increased if these patients suffered various in-hospital complications; (3) on the basis of the CD41a fluorescence of the antibody-stained MPC, some of the aggregates seem to consist of monocytes and platelet-derived extracellular vesicles (EVs); (4) aggregation of monocytes with platelet EV occurred in in vitro experiments; and (5) these experiments demonstrated that monocytes from AMI patients aggregate with both platelets and platelet EVs more efficiently than do monocytes from controls. MPC in AMI patients may play an important role in this pathology. |
| Databáze: | OpenAIRE |
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