Gut microbiota characterization in Chinese patients with alopecia areata
| Autor: | Ying Zhao, Yumei Han, Qinping Yang, Ying Miao, Lijuan Zhou, Sisi Qi, Pan Zhang, Ruiming Hu, Jinghao Lu |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult DNA Bacterial Male China Achromobacter Alopecia Areata Dermatology Gut flora Biochemistry 030207 dermatology & venereal diseases 03 medical and health sciences Young Adult 0302 clinical medicine RNA Ribosomal 16S Megasphaera medicine Humans Molecular Biology Phylogeny biology Lachnospiraceae Alopecia areata biology.organism_classification medicine.disease Healthy Volunteers Gastrointestinal Microbiome 030104 developmental biology Cross-Sectional Studies Early Diagnosis Immunology Cohort 16s rrna gene sequencing Biomarker (medicine) Female Biomarkers |
| Zdroj: | Journal of dermatological science. 102(2) |
| ISSN: | 1873-569X |
| Popis: | Background The gut microbiota is known to play a key role in autoimmune diseases. Objectives To identify and compare the characteristics in the gut microbial composition of patients with alopecia areata (AA) and healthy controls (HCs). Methods In a cross-sectional discovery cohort, we enrolled 33 patients with AA and 35 HCs from the same geographic location in Shanghai, China. The 16S rRNA gene sequencing and bioinformatic analyses were conducted to analyze DNA extracted from the subjects. Results The α-diversity of the AA group demonstrated no statistically significant differences compared with the HC group (P > 0.05). However, the overall gut microbial communities in the AA group were distinct from the HCs (P = 0.0096). We also adopted a random forest model to select three AA-associated OTU biomarkers: OTU1237(Achromobacter), OTU257(Megasphaera), and OTU1784(Lachnospiraceae Incertae Sedis). Conclusion The overall gut microbial composition for AA was distinct from that of HCs. The gut microbial markers we identified may potentially be used for earlier diagnosis and as therapeutic targets. |
| Databáze: | OpenAIRE |
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